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Btbd7导致E-钙黏蛋白表达降低，并预示非小细胞肺癌的预后不良。

Btbd7 contributes to reduced E-cadherin expression and predicts poor prognosis in non-small cell lung cancer.

作者信息

Fan Chuifeng, Miao Yuan, Zhang Xiupeng, Liu Di, Jiang Guiyang, Lin Xuyong, Han Qiang, Luan Lan, Xu Zhonghai, Wang Enhua

机构信息

Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang 110001, China.

出版信息

BMC Cancer. 2014 Sep 24;14:704. doi: 10.1186/1471-2407-14-704.

DOI:10.1186/1471-2407-14-704
PMID:25253020
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4189533/
Abstract

BACKGROUND

Disorders of cell adhesion are critical steps in cancer progression in which varieties of markers including cadherins are involved in.Btbd7 was found to inhibit E-cadherin expression in MDCK cells and play important roles during branching morphogenesis of embryonic salivary glands and lungs. However its function in malignant tumors is largely unknown. The aim of this study is to investigate the clinicopathological significance and possible function of Btbd7 in non-small cell lung cancer.

METHODS

Immunohistochemistry and Western blotting were used to investigate Btbd7 expression in non-small cell lung cancer and lung tissues. The clinicopathological association and the overall survival was analyzed. In vitro experiments were performed using siRNA to investigate the function of Btbd7 in lung cancer cells.

RESULTS

Btbd7 expression was elevated in non-small cell lung cancer tissues compared to normal lung tissues. Increased Btbd7 expression was significantly associated with lymph node metastasis, reduced E-cadherin expression and patients' poor clinical outcome. Downregulation of Btbd7 expression in lung cancer cells by siRNA significantly inhibits cancer cell invasion and effectively restores E-cadherin expression in cancer cell membrane.

CONCLUSIONS

Btbd7 contributes to reduced expression of E-cadherin and may be a promising cancer marker in non-small cell lung cancer.

摘要

背景

细胞黏附紊乱是癌症进展中的关键步骤，多种包括钙黏蛋白在内的标志物参与其中。研究发现Btbd7在MDCK细胞中抑制E-钙黏蛋白表达，并在胚胎唾液腺和肺的分支形态发生过程中发挥重要作用。然而，其在恶性肿瘤中的功能尚不清楚。本研究旨在探讨Btbd7在非小细胞肺癌中的临床病理意义及可能的功能。

方法

采用免疫组织化学和蛋白质印迹法检测非小细胞肺癌组织和肺组织中Btbd7的表达。分析其与临床病理特征的相关性及总体生存率。利用小干扰RNA（siRNA）进行体外实验，研究Btbd7在肺癌细胞中的功能。

结果

与正常肺组织相比，非小细胞肺癌组织中Btbd7表达升高。Btbd7表达增加与淋巴结转移、E-钙黏蛋白表达降低及患者不良临床预后显著相关。通过siRNA下调肺癌细胞中Btbd7的表达可显著抑制癌细胞侵袭，并有效恢复癌细胞膜上E-钙黏蛋白的表达。

结论

Btbd7导致E-钙黏蛋白表达降低，可能是非小细胞肺癌中有前景的癌症标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/13f58ad10a25/12885_2013_4889_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/24f57164ceb9/12885_2013_4889_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/0677925074cf/12885_2013_4889_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/00f95dadd99d/12885_2013_4889_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/3d641a3cca61/12885_2013_4889_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/4fc2dfd2c23b/12885_2013_4889_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/8c89b865f6df/12885_2013_4889_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/13f58ad10a25/12885_2013_4889_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/24f57164ceb9/12885_2013_4889_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/0677925074cf/12885_2013_4889_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/00f95dadd99d/12885_2013_4889_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/3d641a3cca61/12885_2013_4889_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/4fc2dfd2c23b/12885_2013_4889_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/8c89b865f6df/12885_2013_4889_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25d/4189533/13f58ad10a25/12885_2013_4889_Fig7_HTML.jpg

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