Yang Liu, Wang Tiejun, Zhang Jun, Liu Zhonghao, Wang Xuxia
Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan, Shandong, China.
Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong, China.
Cancer Biomark. 2016 Jun 24;17(2):179-85. doi: 10.3233/CBM-160629.
BTB/POZ domain-containing protein 7 (BTBD7) is recognized as a regulatory gene that regulates epithelial cell dynamics and branching morphogenesis. It is also reported for regulating epithelial-mesenchymal transition (EMT) molecules and involved in the process of invasion and metastasis of lung cancer and hepatocellular carcinoma. Slug is a transcriptional factor of EMT which plays a crucial role in the process of primary salivary adenoid cystic carcinoma (SACC). However, the role of BTBD7 in SACC and the correlation with Slug have not been identified. This study investigated the expression of BTBD7 and correlation with Slug, as well as the prognostic significance of BTBD7 in SACC.
The expression of BTBD7 and Slug were examined in ACC-LM and ACC-83 cell lines and immunohistochemically in paraffin embedded tissue specimens from 66 primary SACC patients. Statistical analyses were performed to evaluate the correlation between BTBD7 expression and Slug expression and the prognostic significance of BTBD7 expression.
BTBD7 protein expression was initially verified in ACC-LM and ACC-83 cell lines. The positive rate of BTBD7 expression was 62.1% in SACC to 20% in normal salivary tissues comparatively. BTBD7 expression was significantly correlated with Slug expression in SACC (P< 0.05). Increased BTBD7 expression was significantly associated with the TNM stage, tissue typing, distant metastasis and patients' poor clinical outcome.
Positive expression of BTBD7 in SACC could play an important role in the development of cancer and may serve as a favorable predictor for diagnosis and poor prognosis of patients.
含BTB/POZ结构域蛋白7（BTBD7）被认为是一种调节上皮细胞动态和分支形态发生的调控基因。也有报道称其可调节上皮-间质转化（EMT）分子，并参与肺癌和肝细胞癌的侵袭和转移过程。Slug是EMT的转录因子，在原发性涎腺腺样囊性癌（SACC）过程中起关键作用。然而，BTBD7在SACC中的作用及其与Slug的相关性尚未明确。本研究调查了BTBD7的表达及其与Slug的相关性，以及BTBD7在SACC中的预后意义。
检测了ACC-LM和ACC-83细胞系中BTBD7和Slug的表达，并对66例原发性SACC患者石蜡包埋组织标本进行免疫组织化学检测。进行统计学分析以评估BTBD7表达与Slug表达之间的相关性以及BTBD7表达的预后意义。
最初在ACC-LM和ACC-83细胞系中验证了BTBD7蛋白表达。SACC中BTBD7表达阳性率为62.1%，而正常涎腺组织中为20%。SACC中BTBD7表达与Slug表达显著相关（P<0.05）。BTBD7表达增加与TNM分期、组织类型、远处转移及患者不良临床结局显著相关。
BTBD7在SACC中的阳性表达可能在癌症发展中起重要作用，并且可能作为患者诊断和预后不良的一个良好预测指标。
