Owen Joshua, Grove Philip, Rademeyer Paul, Stride Eleanor
Institute of Biomedical Engineering, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, UK.
Institute of Biomedical Engineering, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, UK
J R Soc Interface. 2014 Nov 6;11(100):20140622. doi: 10.1098/rsif.2014.0622.
The ability to successfully target the delivery of drugs and other therapeutic molecules has been a key goal of biomedical research for many decades. Despite highly promising in vitro results, however, successful translation of targeted drug delivery into clinical use has been extremely limited. This study investigates the significance of the characteristics of whole blood, which are rarely accounted for in vitro assays, as a possible explanation for the poor correlation between in vitro and in vivo experiments. It is shown using two separate model systems employing either biochemical or magnetic targeting that blood causes a substantial reduction in targeting efficiency relative to saline under the same flow conditions. This finding has important implications for the design of targeted drug delivery systems and the assays used in their development.
几十年来，成功实现药物和其他治疗性分子的靶向递送一直是生物医学研究的关键目标。然而，尽管体外实验结果前景十分广阔，但靶向药物递送在临床应用中的成功转化却极为有限。本研究探讨了全血特性的重要性，而体外实验中很少考虑这些特性，这可能是体外和体内实验之间相关性较差的一个原因。在两个独立的模型系统中，分别采用生化靶向或磁靶向，结果表明，在相同流动条件下，相对于生理盐水，血液会导致靶向效率大幅降低。这一发现对靶向药物递送系统的设计及其开发中所使用的检测方法具有重要意义。
