State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (MARC), Tsinghua University, Beijing, 100084, China.
Appl Microbiol Biotechnol. 2015 Feb;99(4):1947-55. doi: 10.1007/s00253-014-6095-7. Epub 2014 Sep 26.
Biofilm formation in drinking water distribution systems (DWDS) has many adverse consequences. Knowledge of microbial community structure of DWDS biofilm can aid in the design of an effective control strategy. However, biofilm bacterial community in real DWDS and the impact of drinking water purification strategy remain unclear. The present study investigated the composition and diversity of biofilm bacterial community in real DWDSs transporting waters with different purification strategies (conventional treatment and integrated treatment). High-throughput Illumina MiSeq sequencing analysis illustrated a large shift in the diversity and structure of biofilm bacterial community in real DWDS. Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Nitrospirae, and Cyanobacteria were the major components of biofilm bacterial community. Proteobacteria (mainly Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria) predominated in each DWDS biofilm, but the compositions of the dominant proteobacterial classes and genera and their proportions varied among biofilm samples. Drinking water purification strategy could shape DWDS biofilm bacterial community. Moreover, Pearson's correlation analysis indicated that Actinobacteria was positively correlated with the levels of total alkalinity and dissolved organic carbon in tap water, while Firmicutes had a significant positive correlation with nitrite nitrogen.
饮用水分配系统 (DWDS) 中的生物膜形成会带来许多不良后果。了解 DWDS 生物膜中的微生物群落结构有助于设计有效的控制策略。然而，实际 DWDS 中的生物膜细菌群落以及饮用水净化策略的影响仍不清楚。本研究调查了输送不同净化策略（常规处理和综合处理）水的实际 DWDS 中生物膜细菌群落的组成和多样性。高通量 Illumina MiSeq 测序分析表明，实际 DWDS 中生物膜细菌群落的多样性和结构发生了很大变化。变形菌门、厚壁菌门、拟杆菌门、放线菌门、硝化螺旋菌门和蓝细菌门是生物膜细菌群落的主要组成部分。在每个 DWDS 生物膜中，变形菌门（主要是α变形菌门、β变形菌门和γ变形菌门）占优势，但优势变形菌门类群和属的组成及其比例在生物膜样本之间有所不同。饮用水净化策略可以塑造 DWDS 生物膜细菌群落。此外，Pearson 相关性分析表明，放线菌与自来水中总碱度和溶解有机碳的水平呈正相关，而 Firmicutes 与亚硝酸盐氮呈显著正相关。
