Hulanicka Magdalena, Garncarz Magdalena, Parzeniecka-Jaworska Marta, Jank Michał
Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska str, 159c, Warsaw, 02-776, Poland.
BMC Vet Res. 2014 Sep 26;10:205. doi: 10.1186/s12917-014-0205-8.
Endocardiosis is the most common heart disease in Dachshunds and is therefore an important cause of cardiac morbidity and death. In recent years we have observed an increasing interest in the development of new genetic and genomic markers of heart disease. The discovery of miRNAs circulating in biofluids such as plasma or serum aroused researchers' interest in using them as potential biomarkers. In the present study we analysed the expression of 9 miRNAs described in literature as being involved in cardiovascular pathology in the plasma of dogs suffering from endocardiosis.
Expression analysis using the Real-time PCR method revealed that two out of nine miRNAs were significantly downregulated: the expression of miR-30b differed between ACVIM stage B and stage A (control) dogs; the expression of mi-133b differed ACVIM stage C and stage A dogs. 5 miRNAs (miR-125, miR-126, miR-21, miR-29b and miR-30b) showed a trend of downregulation in the ACVIM C group. Levels of miR-423 were the same in healthy and diseased dogs. Expression of miR-208a and 208b was not detected.
miR-30b could be a potential biomarker of ACVIM stage B heart failure in Dachshunds with endocardiosis and miR-133b could be a potential biomarker of ACVIM stage C. The lack of expression or lack of significant changes in expression in 7 miRNAs which are potential biomarkers of heart diseases in humans proves that findings from human medicine are not always directly reflected in veterinary medicine.
心内膜炎是腊肠犬最常见的心脏病，因此是心脏发病和死亡的重要原因。近年来，我们观察到人们对心脏病新的遗传和基因组标记物的开发越来越感兴趣。在血浆或血清等生物流体中循环的微小RNA（miRNA）的发现引起了研究人员将其用作潜在生物标志物的兴趣。在本研究中，我们分析了文献中描述的9种参与心血管病理过程的miRNA在患有心内膜炎的犬血浆中的表达情况。
使用实时PCR方法进行的表达分析显示，9种miRNA中有2种显著下调：miR-30b在ACVIM B期和A期（对照）犬之间的表达存在差异；mi-133b在ACVIM C期和A期犬之间的表达存在差异。5种miRNA（miR-125、miR-126、miR-21、miR-29b和miR-30b）在ACVIM C组中呈现下调趋势。miR-423在健康犬和患病犬中的水平相同。未检测到miR-208a和208b的表达。
miR-30b可能是患有心内膜炎的腊肠犬ACVIM B期心力衰竭的潜在生物标志物，miR-133b可能是ACVIM C期的潜在生物标志物。7种在人类中作为心脏病潜在生物标志物的miRNA缺乏表达或表达无显著变化，这证明了人类医学的研究结果并不总是直接反映在兽医学中。
