Department of Veterinary Medicine and Animal Science, University of Milan, Lodi, Italy.
Department of Veterinary Medical Sciences, Alma Mater Studiorum-University of Bologna, Bologna, Italy.
PLoS One. 2022 Dec 27;17(12):e0274724. doi: 10.1371/journal.pone.0274724. eCollection 2022.
Specific microRNAs expressions may accurately characterize different stages of canine myxomatous mitral valve disease. This preliminary pilot study aimed to (1) describe the clinical and echocardiographic parameters of Cavalier King Charles Spaniels affected by myxomatous mitral valve disease at different American College of Veterinary Internal Medicine (ACVIM) stages (B1, B2 and C) and healthy control group (ACVIM A), comparing the parameters collected during the first examination (T0) and the end of the follow-up (T1); (2) assess the association between the values of echocardiographic parameters at T1 and the expression profile of miR-30b-5p at T0. Thirty-five Cavalier King Charles Spaniels (median age 4.29 years and median weight 9 Kg) in different ACVIM stages were included (7 A, 19 B1, 6 B2 and 3 C). Inverse probability weighting analysis was performed to estimate the association of the exposure variable (miR-30b-5p) with the outcome variables (clinical and echocardiographic variables). Time was included as variable. The results pointed out that high levels of plasma miR-30b-5p corresponded to lower values of left ventricular end-diastolic diameter normalized for body weight, end-diastolic and end-systolic volumes indexed for body weight, and left atrium-to aortic root ratio. Hence, higher miR-30b-5p expressions were associated with milder forms of mitral valve disease in our study population. In contrast, the results obtained for the intensity of heart murmur, the mitral regurgitation severity, and the Mitral INsufficiency Echocardiographic score) were not statistically significant. A relationship between high abundance of miR-30b-5p and myxomatous mitral valve disease that appear echocardiographically more stable over time has been demonstrated. In conclusion, Cavalier King Charles Spaniels affected by myxomatous mitral valve disease that at the first cardiologic evaluation showed an upregulation of miR-30b-5p are expected to experience lesser variations on their echocardiographic examination between T0 and T1.
特定的 microRNAs 表达可能准确地表征犬传染性心脏病二尖瓣病的不同阶段。本初步试点研究旨在：(1) 描述不同美国兽医内科学院 (ACVIM) 阶段（B1、B2 和 C）和健康对照组（ACVIM A）受黏液瘤性二尖瓣病影响的骑士查理王小猎犬的临床和超声心动图参数，比较首次检查（T0）和随访结束（T1）时收集的参数；(2) 评估 T1 时超声心动图参数值与 T0 时 miR-30b-5p 表达谱之间的相关性。共纳入 35 只不同 ACVIM 阶段的骑士查理王小猎犬（中位年龄 4.29 岁，中位体重 9kg）（7 只 A 组、19 只 B1 组、6 只 B2 组和 3 只 C 组）。进行逆概率加权分析以估计暴露变量（miR-30b-5p）与结局变量（临床和超声心动图变量）之间的相关性。时间被包括为变量。结果表明，血浆 miR-30b-5p 水平高与左心室舒张末期直径按体重标准化、舒张末期和收缩末期容积按体重指数、左心房至主动脉根部比值减小相对应。因此，在我们的研究人群中，较高的 miR-30b-5p 表达与二尖瓣疾病的轻度形式相关。相比之下，心脏杂音强度、二尖瓣反流严重程度和二尖瓣关闭不全超声心动图评分的结果没有统计学意义。在时间上，miR-30b-5p 高丰度与黏液瘤性二尖瓣病之间的关系已被证明更为稳定。总之，在首次心脏评估时显示 miR-30b-5p 上调的受黏液瘤性二尖瓣病影响的骑士查理王小猎犬，预计在 T0 和 T1 之间的超声心动图检查中会出现较小的变化。
