Mehta-D'souza Padmaja
Oklahoma Medical Research Center, 825 N.E. 13th Street, Oklahoma City, OK, 73104, USA,
Methods Mol Biol. 2015;1207:105-14. doi: 10.1007/978-1-4939-1396-1_7.
Surface Plasmon Resonance (SPR) instruments, like the BIAcore 3000, are useful for studying the binding between macromolecules in real time. The high sensitivity and low sample consumption in the BIAcore enables the measurement of rapid kinetics and low affinities characteristic of many biological interactions. This chapter describes the affinity measurement of Galectins-1, -2, and -3 and their glycoside ligands using this approach.
表面等离子体共振（SPR）仪器，如BIAcore 3000，可用于实时研究大分子之间的结合。BIAcore的高灵敏度和低样品消耗能够测量许多生物相互作用所特有的快速动力学和低亲和力。本章描述了使用这种方法对半乳糖凝集素-1、-2和-3及其糖苷配体进行亲和力测量。
