Gittens Beatrice R, Wright Rachael D, Cooper Dianne
William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, Charterhouse Square, EC1M 6BQ, London, UK.
Methods Mol Biol. 2015;1207:133-51. doi: 10.1007/978-1-4939-1396-1_9.
Numerous protocols exist for investigating leukocyte recruitment both in vitro and in vivo. Here we describe three of these methods; an in vitro flow chamber assay, intravital microscopy, and zymosan-induced peritonitis, and give details as to how they can be used to study the actions of galectins on this crucial process.
现有许多用于在体外和体内研究白细胞募集的实验方案。在此,我们描述其中三种方法:体外流动腔室分析、活体显微镜检查和酵母聚糖诱导的腹膜炎,并详细说明如何使用它们来研究半乳糖凝集素在这一关键过程中的作用。