William Harvey Research Institute, Barts and The London School of Medicine, Queen Mary University of London, London, UK.
Methods Mol Biol. 2022;2442:581-601. doi: 10.1007/978-1-0716-2055-7_31.
Numerous protocols exist for investigating leukocyte recruitment and clearance both in vitro and in vivo. Here we describe an in vitro flow chamber assay typically used for studying the mechanisms underpinning leukocyte movement through the endothelium and zymosan-induced peritonitis, an acute in vivo model of inflammation that enables both leukocyte trafficking and clearance to be monitored. Insight is given as to how these models can be used to study the actions of galectins on the inflammatory process.
有许多研究白细胞募集和清除的方案，包括体外和体内研究。本文介绍了一种常用的体外流动室分析方法，用于研究白细胞穿过内皮细胞的机制，以及酵母聚糖诱导的腹膜炎，后者是一种急性体内炎症模型，可同时监测白细胞迁移和清除。本文还介绍了如何使用这些模型来研究半乳糖凝集素对炎症过程的作用。
