沙门氏菌中Stn蛋白的研究；膜组成与完整性的调节因子。

Study of the Stn protein in Salmonella; a regulator of membrane composition and integrity.

作者信息

Nakano Masayuki, Yamasaki Eiki, Moss Joel, Hirayama Toshiya, Kurazono Hisao

机构信息

Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, 1-12-4, Sakamoto, Nagasaki, 852-8523, Japan,

出版信息

Methods Mol Biol. 2015;1225:127-38. doi: 10.1007/978-1-4939-1625-2_9.

DOI:10.1007/978-1-4939-1625-2_9

PMID:25253253

Abstract

Our studies were undertaken to develop new insights into the function of the Salmonella Stn protein. An analysis of total cell membrane protein fraction suggested the possibility that Stn associates with OmpA. This possibility was confirmed by immunogold labeling using anti-OmpA antibody and far-western blotting. From these results, we conclude that Stn regulates membrane composition and integrity in Salmonella.

摘要

我们开展了研究，以深入了解沙门氏菌Stn蛋白的功能。对全细胞膜蛋白组分的分析表明，Stn可能与OmpA相关联。使用抗OmpA抗体的免疫金标记和远缘Western印迹证实了这种可能性。从这些结果来看，我们得出结论，Stn调节沙门氏菌的膜组成和完整性。