College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon, Korea.
Electrophoresis. 2014 Mar;35(6):888-94. doi: 10.1002/elps.201300199. Epub 2014 Jan 13.
Salmonella enterica serovar Gallinarum (SG) is an important pathogen that causes fowl typhoid in chickens. In order to investigate SG outer membrane proteins (OMPs) as potential vaccine candidate proteins, we established a proteomic map and database of antigenic SG-OMPs. A total of 174 spots were detected by 2DE. Twenty-two antigen-reactive spots were identified as nine specific proteins using PMF. OmpA was the most abundant protein among all of the identified OMPs, and it exhibited seven protein species. We conducted Western blot analysis for the SG-OMPs in order to determine which proteins were cross-reactive to the serovars Salmonella Enteritidis, Salmonella Typhimurium, and SG. Our results indicated that OmpA was considered to be an antigenic cross-reactive protein among the three serovars. This study sheds new light on our understanding of cross-protection among Salmonella serovars.
肠炎沙门氏菌血清型鸡白痢（SG）是一种重要的病原体，可引起鸡伤寒。为了研究 SG 外膜蛋白（OMPs）作为潜在的疫苗候选蛋白，我们建立了一个 SG-OMPs 的蛋白质组图谱和数据库。2DE 检测到 174 个斑点。使用 PMF 鉴定了 22 个抗原反应斑点为 9 种特定蛋白质。OmpA 是所有鉴定出的 OMPs 中含量最丰富的蛋白质，有七种蛋白形式。我们对 SG-OMPs 进行了 Western blot 分析，以确定哪些蛋白质与肠炎沙门氏菌、鼠伤寒沙门氏菌和 SG 血清型发生交叉反应。结果表明，OmpA 被认为是三种血清型之间的一种抗原交叉反应蛋白。本研究为我们理解沙门氏菌血清型之间的交叉保护提供了新的认识。
