Riesco Marta F, Valcarce David G, Alfonso Javier, Herráez M Paz, Robles Vanesa
Department of Molecular Biology and Cell Biology Area, University of León, León, Spain.
Department of Mechanical, Computing, and Aerospace Engineering, University of León, León, Spain.
Biol Reprod. 2014 Nov;91(5):114. doi: 10.1095/biolreprod.114.121491. Epub 2014 Sep 24.
The possibility of generating primordial germ cells (PGCs) in vitro from noncommitted embryonic cells represents an extremely useful tool in current research. Primordial germ cell in vitro differentiation has been successfully reported in mammals. However, contrary to fish, PGC specification in mammals is an inductive mechanism. This study is the first to date to describe a rapid method for PGC in vitro differentiation in teleosts. Primordial germ cell-like cells were characterized by several lines of evidence, including gene expression, cell complexity, size, and image analysis for the quantification of fluorescence under vasa promoter. Moreover, differentiated cells were able to colonize the genital ridge after transplantation. Differentiation treatments increased the number of PGCs in culture, causing differentiation of cells rather than inducing their proliferation. These results open up the possibility of differentiating genetically modified embryonic cells to PGC-like cells to ensure their transmission to the progeny and could be crucial for an in-depth understanding of germline differentiation in teleosts.
从未分化的胚胎细胞体外生成原始生殖细胞（PGCs）的可能性是当前研究中一种极其有用的工具。在哺乳动物中已成功报道了原始生殖细胞的体外分化。然而，与鱼类不同，哺乳动物中的PGC特化是一种诱导机制。本研究是迄今为止首个描述硬骨鱼中PGC体外快速分化方法的研究。原始生殖细胞样细胞通过多条证据进行了表征，包括基因表达、细胞复杂性、大小以及在vasa启动子下用于荧光定量的图像分析。此外，分化后的细胞在移植后能够定殖到生殖嵴。分化处理增加了培养物中PGC的数量，导致细胞分化而非诱导其增殖。这些结果为将转基因胚胎细胞分化为PGC样细胞以确保其传递给后代开辟了可能性，并且对于深入了解硬骨鱼中的生殖系分化可能至关重要。
