中国人群中热休克蛋白70-2的多态性与肠皮肤瘘

Polymorphism of heat shock protein 70-2 and enterocutaneous fistula in Chinese population.

作者信息

Chen Jun, Ren Jian-An, Han Gang, Gu Guo-Sheng, Wang Ge-Fei, Wu Xiu-Wen, Zhou Bo, Hu Dong, Wu Yin, Zhao Yun-Zhao, Li Jie-Shou

机构信息

Jun Chen, Jian-An Ren, Guo-Sheng Gu, Ge-Fei Wang, Xiu-Wen Wu, Yun-Zhao Zhao, Jie-Shou Li, Department of Surgery, the Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu Province, China.

出版信息

World J Gastroenterol. 2014 Sep 21;20(35):12559-65. doi: 10.3748/wjg.v20.i35.12559.

DOI:10.3748/wjg.v20.i35.12559

PMID:25253958

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4168091/

Abstract

AIM

To investigate whether the heat shock protein 70-2 (HSP70-2) polymorphism is associated with enterocutaneous fistulas in a Chinese population.

METHODS

This study included 131 patients with enterocutaneous/enteroatmospheric fistulas. Patients with inflammatory bowel disease or other autoimmune diseases were excluded from this study. All patients with enterocutaneous/enteroatmospheric fistulas were followed up for three months to observe disease recurrence. In addition, a total of 140 healthy controls were also recruited from the Jinling Hospital, matched according to the sex and age of the patient population. Genomic DNA was extracted from peripheral blood from each participant. The HSP70-2 restriction fragment length polymorphism related to the polymorphic PstI site at position 1267 was characterized by polymerase chain reaction (PCR). First PCR amplification was carried out, and then PCR products were digested with PstI restriction enzyme. The DNA lacking the polymorphic PstI site within HSP70-2 generates a product of 1117 bp in size (allele A), whereas the HSP70-2 PstI polymorphism produces two fragments of 936 bp and 181 bp in size (allele B).

RESULTS

The frequency of the HSP70-2 PstI polymorphism did not differ between patients and controls; however, the A allele was more predominant in patients with enterocutaneous fistulas than in controls (60.7% vs 51.4%, P = 0.038, OR = 1.425, 95%CI: 1.019-1.994). Sixty-one patients were cured by a definitive operation, drainage operation, or percutaneous drainage while 52 patients were cured by nonsurgical treatment. There was no significant difference in the frequency of the HSP70-2 PstI polymorphism between the patients who had surgery compared to those who did not (P = 0.437, OR = 1.237, 95%CI: 0.723-2.117). Moreover, 11 patients refused any treatment for economic reasons or tumor burden, and 7 patients with enterocutaneous fistulas (5.8%) died during the follow-up period. However, there was no significant difference in the frequency of the HSP70-2 PstI polymorphism between the patients who survived compared to those who died (P = 0.403, OR = 0.604, 95%CI: 0.184-1.986).

CONCLUSION

The A allele of the HSP70-2 PstI polymorphism was associated with enterocutaneous fistulas in this Chinese population.

摘要

目的

研究热休克蛋白70-2（HSP70-2）基因多态性与中国人群肠皮肤瘘的相关性。

方法

本研究纳入131例肠皮肤/肠腔瘘患者。排除患有炎症性肠病或其他自身免疫性疾病的患者。对所有肠皮肤/肠腔瘘患者进行为期三个月的随访，观察疾病复发情况。此外，还从金陵医院招募了140名健康对照者，根据患者的性别和年龄进行匹配。从每个参与者的外周血中提取基因组DNA。通过聚合酶链反应（PCR）对与1267位多态性PstI位点相关的HSP70-2限制性片段长度多态性进行鉴定。首先进行PCR扩增，然后用PstI限制性酶消化PCR产物。HSP70-2内缺乏多态性PstI位点的DNA产生大小为1117 bp的产物（等位基因A），而HSP70-2 PstI多态性产生大小为936 bp和181 bp的两个片段（等位基因B）。

结果

患者与对照组之间HSP70-2 PstI多态性的频率没有差异；然而，肠皮肤瘘患者中A等位基因比对照组更占优势（60.7%对51.4%，P = 0.038，OR = 1.425，95%CI：1.019 - 1.994）。61例患者通过确定性手术、引流手术或经皮引流治愈，52例患者通过非手术治疗治愈。接受手术的患者与未接受手术的患者之间HSP70-2 PstI多态性的频率没有显著差异（P = 0.437，OR = 1.237，95%CI：0.723 - 2.117）。此外，11例患者因经济原因或肿瘤负担拒绝任何治疗，7例肠皮肤瘘患者（5.8%）在随访期间死亡。然而，存活患者与死亡患者之间HSP70-2 PstI多态性的频率没有显著差异（P = 0.403，OR = 0.604，95%CI：0.184 - 1.986）。