Shikowitz M J, Galli R, Bandyopadhyay D, Hoory S
Department of Otolaryngology and Communicative Disorders, Long Island Jewish Medical Center, New Hyde Park, NY 11042.
Arch Otolaryngol Head Neck Surg. 1989 Jul;115(7):845-7. doi: 10.1001/archotol.1989.01860310083028.
Hematoporphyrin derivative and its newly purified form, dihematoporphyrin ether, have been shown to localize selectively in malignant tissues and virally induced papillomas. Its use as a probe to distinguish tumors from normal tissues has been largely based on its fluorescence when activated by UV light. These findings are largely subjective, and a direct correlation to its use as a photosensitizing agent to selectively kill transformed cells when activated by an appropriate wavelength of light could not be made. The efficacy of dihematoporphyrin ether photodynamic therapy to selectively kill papillomavirus-transformed cells is based on the increased localization of dihematoporphyrin ether within these tissues as compared with normal tissues. Using cottontail rabbit papillomavirus, cutaneous papillomas were induced on the backs of Dutch belted rabbits. Dihematoporphyrin ether was labeled with indium 111 and intravenously injected into the rabbits. The animals were scanned twice daily for indium 111 activity on a large-field-of-view gamma camera. At 50 hours after injection, the rabbits were killed and papillomas, skin, and major organs collected for biodistribution studies. The results of this study and their relationship to dihematoporphyrin ether photodynamic therapy for the treatment of virally induced papilloma disease is discussed.
血卟啉衍生物及其新纯化形式二血卟啉醚已被证明可选择性地定位于恶性组织和病毒诱导的乳头瘤中。它作为区分肿瘤与正常组织的探针，很大程度上是基于其在紫外光激活下的荧光。这些发现很大程度上是主观的，并且无法直接将其与作为光敏剂在适当波长的光激活下选择性杀死转化细胞的用途联系起来。二血卟啉醚光动力疗法选择性杀死乳头瘤病毒转化细胞的功效基于与正常组织相比，二血卟啉醚在这些组织中的定位增加。使用棉尾兔乳头瘤病毒，在荷兰带兔的背部诱导皮肤乳头瘤。二血卟啉醚用铟111标记并静脉注射到兔子体内。每天在大视野γ相机上对动物进行两次铟111活性扫描。注射后50小时，处死兔子并收集乳头瘤、皮肤和主要器官进行生物分布研究。讨论了本研究的结果及其与二血卟啉醚光动力疗法治疗病毒诱导的乳头瘤病的关系。
