Antunes Marina Venzon, da Silva Cássia Franciele Rosa, Finger Marcéli Andressa, Moore Christine, Linden Rafael
*Institute of Health Sciences, Universidade Feevale, Novo Hamburgo, Brazil; and †Immunalysis Corporation, Pomona, CA.
Ther Drug Monit. 2015 Jun;37(3):405-7. doi: 10.1097/FTD.0000000000000140.
To determine cotinine levels in hair samples collected from smokers and estimate their relationship with nicotine intake and nicotine dependence class according to the Fagerström dependence questionnaire.
Sixty-nine volunteers participated. They gave hair samples and answered questionnaires covering information related to smoking habits. Cotinine levels were determined by enzyme immunoassay after extraction from 20 mg of hair. Calibrators for the range 100 to 12,800 pg/mg were used.
Mean cotinine concentration was 2070.9 pg/mg (range, 469.4-10,188.6 pg/mg). There were significant correlations between cotinine levels in hair and the questionnaire results (rs = 0.325, P = 0.018) and with the number of cigarettes smoked in a day (rs = 0.717, P < 0.001). Although the correlation between questionnaire results and the number of cigarettes smoked per day was significant (rs = 0.565, P < 0.01), it was weaker than the association between cotinine levels and number of cigarettes smoked. Cotinine levels were lower in the group that smoked from 1 to 5 cigarettes per day (1104.1 ng/mg) compared with the other groups.
Cotinine levels were more highly correlated with nicotine exposure than with the Fagerström questionnaire scores. It was found that interference from exogenous sources of contamination, such as hair dyes, is a limitation to estimate nicotine intake from hair analysis.
测定从吸烟者收集的头发样本中的可替宁水平，并根据法格斯特龙依赖问卷评估其与尼古丁摄入量及尼古丁依赖等级的关系。
69名志愿者参与。他们提供头发样本并回答涵盖吸烟习惯相关信息的问卷。从20毫克头发中提取后，通过酶免疫测定法测定可替宁水平。使用了100至12,800 pg/mg范围内的校准物。
可替宁平均浓度为2070.9 pg/mg（范围为469.4 - 10,188.6 pg/mg）。头发中的可替宁水平与问卷结果（rs = 0.325，P = 0.018）以及每日吸烟量（rs = 0.717，P < 0.001）之间存在显著相关性。尽管问卷结果与每日吸烟量之间的相关性显著（rs = 0.565，P < 0.01），但它比可替宁水平与吸烟量之间的关联更弱。与其他组相比，每天吸1至5支烟的组中可替宁水平较低（1104.1 ng/mg）。
可替宁水平与尼古丁暴露的相关性高于与法格斯特龙问卷得分的相关性。研究发现，染发剂等外源性污染源的干扰是通过头发分析估计尼古丁摄入量的一个限制因素。
