Nardini Giordano, Barbarossa Andrea, Dall'Occo Andrea, Di Girolamo Nicola, Cagnardi Petra, Magnone William, Bielli Mattia, Roncada Paola, Zaghini Anna
Clinica Veterinaria Modena Sud, Piazza dei Tintori 1, 41057 Spilamberto, MO, Italy.
Am J Vet Res. 2014 Oct;75(10):918-23. doi: 10.2460/ajvr.75.10.918.
To determine the pharmacokinetics of cefovecin sodium after SC administration to Hermann's tortoises (Testudo hermanni).
23 healthy adult Hermann's tortoises (15 males and 8 females).
Cefovecin (8.0 mg/kg) was injected once in the subcutis of the neck region of Hermann's tortoises, and blood samples were obtained at predetermined time points. Plasma cefovecin concentrations were measured via ultraperformance liquid chromatography coupled to tandem mass spectrometry, and pharmacokinetic parameters were calculated with a noncompartmental model. Plasma protein concentration was quantified, and the percentage of cefovecin bound to protein was estimated with a centrifugation technique.
Cefovecin was absorbed rapidly, reaching maximum plasma concentrations between 35 minutes and 2 hours after administration, with the exception of 1 group, in which it was reached after 4 hours. The mean ± SD time to maximum concentration was 1.22 ± 1.14 hours; area under the concentration-time curve was 220.35 ± 36.18 h•μg/mL The mean protein-bound fraction of cefovecin ranged from 41.3% to 47.5%. No adverse effects were observed.
Administration of a single dose of cefovecin SC appeared to be well-tolerated in this population of tortoises. Results of pharmacokinetic analysis indicated that the 2-week dosing interval suggested for dogs and cats cannot be considered effective in tortoises; however, further research is needed to determine therapeutic concentrations of the drug and appropriate dose ranges.
确定头孢维星钠皮下注射给赫尔曼陆龟（Testudo hermanni）后的药代动力学。
23只健康成年赫尔曼陆龟（15只雄性和8只雌性）。
将头孢维星（8.0 mg/kg）一次性注射到赫尔曼陆龟颈部皮下，在预定时间点采集血样。通过超高效液相色谱-串联质谱法测定血浆中头孢维星浓度，并用非房室模型计算药代动力学参数。定量血浆蛋白浓度，采用离心技术估算头孢维星与蛋白结合的百分比。
头孢维星吸收迅速，给药后35分钟至2小时达到血浆最大浓度，但有1组在4小时后达到。达到最大浓度的平均±标准差时间为1.22±1.14小时；浓度-时间曲线下面积为220.35±36.18 h•μg/mL。头孢维星与蛋白结合的平均比例为41.3%至47.5%。未观察到不良反应。
在这群陆龟中，单剂量皮下注射头孢维星似乎耐受性良好。药代动力学分析结果表明，建议用于犬猫的2周给药间隔对陆龟无效；然而，需要进一步研究以确定该药物的治疗浓度和合适的剂量范围。
