VRC, Veterinary Reference Centre, Torino, Italy.
The Royal (Dick) School of Veterinary Studies and the Roslin Institute, University of Edinburgh, Edinburgh, UK.
Vet Res Commun. 2023 Dec;47(4):2279-2284. doi: 10.1007/s11259-023-10138-6. Epub 2023 May 13.
Deslorelin acetate is a gonadotropin-releasing hormone agonist formulated in a controlled-release subcutaneous implant and designed for reversible suppression of testosterone production in dogs. It has also been demonstrated to be effective in other animal species, but no data on its effectiveness in male land tortoises are available. This study aimed to evaluate the effect of a 4.7-mg deslorelin acetate implant on serum testosterone concentrations in male Hermann's (Testudo hermanni sp.) and Greek (Testudo graeca sp.) tortoises. Twenty adult male tortoises housed under the same environmental conditions were enrolled for the study and randomly assigned to a treatment (D, n = 10) or a control (C, n = 10) group. Starting in May, males from the D group were implanted with a 4.7-mg deslorelin acetate device, whereas males from the C group did not receive any treatment. Blood samples were collected once immediately before implant application (S0-May) and at 15 days (S1-June), 2 (S2-July), and 5 (S3-October) months after application. Serum testosterone at each sampling time was measured through a solid-phase, enzyme-labeled, competitive chemiluminescent immunoassay. Median serum testosterone concentrations were not significantly different between the two groups in all sampling times, and no interaction between treatment and sampling time was observed. The present study, therefore, suggests that a single treatment with a 4.7-mg deslorelin acetate implant has no effect on testosterone circulation in male Hermann's and Greek tortoises during the following 5 months.
醋酸地洛瑞林是一种促性腺激素释放激素激动剂，以控释皮下植入物的形式配制，旨在可逆抑制犬的睾酮产生。它已被证明在其他动物物种中也有效，但在雄性陆龟中尚无其有效性的数据。本研究旨在评估 4.7 毫克醋酸地洛瑞林植入物对雄性赫曼陆龟（Testudo hermanni sp.）和希腊陆龟（Testudo graeca sp.）血清睾酮浓度的影响。20 只生活在相同环境条件下的成年雄性陆龟被纳入研究并随机分为治疗（D 组，n = 10）或对照（C 组，n = 10）组。从 5 月开始，D 组的雄性被植入 4.7 毫克醋酸地洛瑞林装置，而 C 组的雄性未接受任何治疗。在植入应用前立即（S0-5 月）和应用后 15 天（S1-6 月）、2 个月（S2-7 月）和 5 个月（S3-10 月）各采集一次血样。通过固相、酶标记、竞争性化学发光免疫测定法测量每个采样时间的血清睾酮。在所有采样时间，两组的血清睾酮浓度中位数均无显著差异，且治疗与采样时间之间无交互作用。因此，本研究表明，在接下来的 5 个月中，单次使用 4.7 毫克醋酸地洛瑞林植入物对雄性赫曼陆龟和希腊陆龟的睾酮循环没有影响。
