Kohl A, Volk H D, Diezel W, Buntrock P, Diamantstein T
Clinic of Paediatrics, Humboldt-Universität Berlin.
Int J Immunopharmacol. 1989;11(3):237-40. doi: 10.1016/0192-0561(89)90160-4.
To determine the role of T-cell subsets in wound healing, we studied the granulation tissue proliferation after depletion of CD4 or CD8 positive cells. Granulation tissue proliferation in CD8-diminished AB mice was significantly higher than in the control group as a result of depleted suppressor cell activity. The CD4-depleted mice produced granulation tissue in less than 30% of the control group. To investigate the role of dipeptidylpeptidase IV on CD4 positive cells in wound healing we used Lys-[Z(NO2)]-Pro, a chemically modified dipeptide which may result by degradation of polypeptides by this peptidase. It was possible to restore the diminished capability of granulation tissue proliferation in CD4-depleted mice by a single treatment with the dipeptide Lys-[Z(NO2)]-Pro. Our results suggest that: (i) the CD4 positive T-helper subset regulates wound healing, and (ii) products of degradation by dipeptidylpeptidase IV may bypass T-helper cell function.
为了确定T细胞亚群在伤口愈合中的作用，我们研究了CD4或CD8阳性细胞耗竭后肉芽组织的增殖情况。由于抑制细胞活性的耗竭，CD8减少的AB小鼠的肉芽组织增殖明显高于对照组。CD4耗竭的小鼠产生的肉芽组织不到对照组的30%。为了研究二肽基肽酶IV对伤口愈合中CD4阳性细胞的作用，我们使用了Lys-[Z(NO2)]-Pro，一种化学修饰的二肽，它可能是由该肽酶降解多肽产生的。通过用二肽Lys-[Z(NO2)]-Pro单次处理，可以恢复CD4耗竭小鼠中减弱的肉芽组织增殖能力。我们的结果表明：(i) CD4阳性T辅助亚群调节伤口愈合，以及(ii) 二肽基肽酶IV的降解产物可能绕过T辅助细胞功能。
