Ermak T H, Steger H J, Heyworth M F
Intestinal Immunology Research Center, Veterans Administration Medical Center, San Francisco, California 94121.
Immunol Lett. 1988 Mar;17(3):205-10. doi: 10.1016/0165-2478(88)90030-2.
The effect of in vivo administration of anti-L3T4 monoclonal antibody on the generation of an L3T4-/Ly-2- (CD4-/CD8-) population of Thy-1.2+ cells was examined in Peyer's patches of mice by two-color flow cytometry. Female BALB/c mice aged 8 wk were given 4-6 weekly injections of either anti-L3T4 (1 mg/wk) or phosphate-buffered saline (control group), and dispersed Peyer's patch cells analyzed for the presence and absence of L3T4 and Ly-2 on Thy-1.2+ cells. In anti-L3T4 treated mice, L3T4-/Ly-2- T cells accounted for 25-30% of the Thy-1.2+ cells, whereas in control mice these cells represented only 3-4% of the T cells. The remaining 70-75% of the Thy-1.2+ cells after anti-L3T4 treatment were Ly-2+ and not L3T4+. The L3T4-/Ly-2- population of Thy-1.2+ cells is a novel subset which has not been previously found in Peyer's patches and is amplified when helper T cells are depleted.
通过双色流式细胞术，在小鼠派尔集合淋巴结中检测了体内给予抗L3T4单克隆抗体对Thy-1.2+细胞中L3T4-/Ly-2-（CD4-/CD8-）细胞群产生的影响。给8周龄的雌性BALB/c小鼠每周注射4 - 6次抗L3T4（1毫克/周）或磷酸盐缓冲盐水（对照组），然后对分散的派尔集合淋巴结细胞进行分析，检测Thy-1.2+细胞上L3T4和Ly-2的有无。在接受抗L3T4治疗的小鼠中，L3T4-/Ly-2- T细胞占Thy-1.2+细胞的25 - 30%，而在对照小鼠中，这些细胞仅占T细胞的3 - 4%。抗L3T4治疗后，其余70 - 75%的Thy-1.2+细胞是Ly-2+而非L3T4+。Thy-1.2+细胞中的L3T4-/Ly-2-细胞群是一个新的亚群，以前在派尔集合淋巴结中未发现，当辅助性T细胞被耗尽时会扩增。
