Grochocki Wojciech, Markuszewski Michał J, Quirino Joselito P
Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdansk, Gdansk, Poland.
Electrophoresis. 2015 Jan;36(1):135-43. doi: 10.1002/elps.201400416. Epub 2014 Nov 7.
Multidimensional separation where two or more orthogonal displacement mechanisms are combined is a promising approach to increase peak capacity in CE. The combinations allow dramatic improvement of analytical performance since the total peak capacity is given by a product of the peak capacities of all methods. The initial reports were concentrated on the construction of effective connections between capillaries for 2D analysis. Today, 2D and 3D CE systems are now able to separate real complex biological or environmental mixtures with good repeatability, improved resolution with minimal loss of sample. This review will present the developments in the field of multidimensional CE during the last 15 years. The endeavors in this specific field were on the development of interfaces, interface-free techniques including integrated separations, microdevices, and on-line sample concentration techniques to improve detection sensitivity.
将两种或更多种正交位移机制相结合的多维分离是提高毛细管电泳峰容量的一种很有前景的方法。由于总峰容量由所有方法的峰容量之积给出，所以这些组合能显著改善分析性能。最初的报道集中在用于二维分析的毛细管之间有效连接的构建上。如今，二维和三维毛细管电泳系统现在能够以良好的重复性分离真正复杂的生物或环境混合物，在样品损失最小的情况下提高分辨率。本综述将介绍过去15年中多维毛细管电泳领域的发展情况。这一特定领域的努力集中在接口的开发、包括集成分离、微型器件在内的无接口技术以及在线样品浓缩技术，以提高检测灵敏度。
