Kohl Felix J, Sánchez-Hernández Laura, Neusüß Christian
Department of Chemistry, Aalen University, Aalen, Germany.
Electrophoresis. 2015 Jan;36(1):144-58. doi: 10.1002/elps.201400368. Epub 2014 Nov 12.
The analysis of complex samples requires powerful separation techniques. Here, 2D chromatographic separation techniques (e.g. LC-LC, GC-GC) are increasingly applied in many fields. Electrophoretic separation techniques show a different selectivity in comparison to LC and GC and very high separation efficiency. Thus, 2D separation systems containing at least one CE-based separation technique are an interesting alternative featuring potentially a high degree of orthogonality. However, the generally small volumes and strong electrical fields in CE require special coupling techniques. These technical developments are reviewed in this work, discussing benefits and drawbacks of offline and online systems. Emphasis is placed on the design of the systems, their coupling, and the detector used. Moreover, the employment of strategies to improve peak capacity, resolution, or sensitivity is highlighted. Various applications of 2D separations with CE are summarized.
对复杂样品的分析需要强大的分离技术。在此，二维色谱分离技术（如液相色谱-液相色谱、气相色谱-气相色谱）在许多领域中得到越来越广泛的应用。与液相色谱和气相色谱相比，电泳分离技术具有不同的选择性和非常高的分离效率。因此，包含至少一种基于毛细管电泳的分离技术的二维分离系统是一种有趣的替代方案，可能具有高度的正交性。然而，毛细管电泳中通常较小的体积和强电场需要特殊的联用技术。本文对这些技术发展进行了综述，讨论了离线和在线系统的优缺点。重点在于系统的设计、它们的联用以及所使用的检测器。此外，还强调了提高峰容量、分辨率或灵敏度的策略的应用。总结了毛细管电泳二维分离的各种应用。
