Maier T, Holda J H, Claman H N
Department of Microbiology, University of Colorado School of Medicine, Denver 80262.
J Immunol. 1989 Jul 15;143(2):491-8.
Natural suppressor (NS) cells are potent, Ag nonspecific, MHC-unrestricted inhibitors of immune responses. Murine NS activity is found in several situations, including adult bone marrow (BM) and neonatal/newborn spleen, and spleen following total lymphoid irradiation, after BM transplantation and after cyclophosphamide (CY) treatment. Using three of these situations (adult BM, newborn spleen, and spleen after CY treatment), the strain distribution of NS cell activity was assessed. A wide variation in potency is seen in both naturally occurring (adult BM and newborn spleen) and induced (after CY treatment) NS cell activity. Up to 10-fold differences in NS activity are seen between high and low NS strains. This reflects an intrinsic genetic variation between mouse strains in both naturally occurring and CY-induced NS cell activity. Thus, a strain with high NS activity at birth, has high NS activity in its BM as an adult and in its spleen after CY treatment. Of the strains tested, B10.D2 has the highest NS cell activity while BALB/c has the lowest, and the F1 between these two strains is intermediate in NS activity. Finally, the NS cell activity from all strains tested required IFN-gamma for expression of its inhibitory activity.
自然抑制（NS）细胞是免疫反应的强效、抗原非特异性、MHC非限制性抑制剂。在多种情况下可发现小鼠的NS活性，包括成年骨髓（BM）、新生/新生儿脾脏，以及全淋巴照射后、骨髓移植后和环磷酰胺（CY）治疗后的脾脏。利用其中三种情况（成年BM、新生脾脏和CY治疗后的脾脏），评估了NS细胞活性的品系分布。在天然存在的（成年BM和新生脾脏）和诱导产生的（CY治疗后）NS细胞活性中，都观察到效力存在很大差异。高NS活性品系和低NS活性品系之间的NS活性差异高达10倍。这反映了小鼠品系在天然存在的和CY诱导的NS细胞活性方面存在内在遗传差异。因此，出生时NS活性高的品系，成年后其BM和CY治疗后的脾脏中NS活性也高。在所测试的品系中，B10.D2的NS细胞活性最高，而BALB/c的NS细胞活性最低，这两个品系之间的F1代NS活性处于中间水平。最后，所有测试品系的NS细胞活性都需要γ干扰素来表达其抑制活性。
