Willers J M, Sluis E
Ann Immunol (Paris). 1975 Apr;126(3):267-79.
A single injection of cyclophosphamide (CY) (300 mg/kg) 8 h prior to immunization results in a complete suppression of the antibody formation in mice. The effect of CY on the number and activity of the surviving cells in the lymphoid organs was studied. For all organs tested there was a decrease in cell numbers to 10% or less till day 3, followed by an increase in cell numbers in bone marrow, spleen and lymph nodes. The increase in cell numbers in the thymus was delayed till after day 11. The bone marrow reached normal levels on day 7. The spleen surpassed the normal levels on day 11, reaching cell numbers which were 50% over the normal ones. For the thymus cells this was found only on day 21. The numbers of lymph node cells remained low and reached normal levels or higher only when the mice were stimulated after CY treatment. This effect of stimulation was further seen in the spleen cell numbers, where after stimulation with antigen in Freund's complete adjuvant (FCA) or only FCA cell numbers of 200% of normal values were obtained. The suppression of antibody formation by CY could be restored by cells from spleen, lymph nodes, Peyer's patches or thymus and bone marrow, indicating a defect in both B and T cells caused by CY treatment. The target cell of CY treatment was studied by reconstitution of anergic mice with spleen or lymph node cells of CY treated mice with or without addition of normal bone marrow and thymus cells. This revealed that although the B cells is the main target cell of CY action, the impact on the T cell population is also considerable. When however CY treatment is followed by antigenic stimulation, there is a steep increase in the activity of the lymph node cells when bone marrow cells are added. This phenomenon resembles the education of thymus cells in the spleen of lethally irradiated mice in the presence of antigen.
在免疫前8小时单次注射环磷酰胺（CY）（300毫克/千克）可导致小鼠抗体形成完全受到抑制。研究了CY对淋巴器官中存活细胞数量和活性的影响。对于所有测试器官，直到第3天细胞数量减少至10%或更低，随后骨髓、脾脏和淋巴结中的细胞数量增加。胸腺中细胞数量的增加延迟至第11天之后。骨髓在第7天达到正常水平。脾脏在第11天超过正常水平，细胞数量比正常水平高出50%。胸腺细胞仅在第21天出现这种情况。淋巴结细胞数量一直较低，仅在CY处理后刺激小鼠时才达到正常水平或更高。在脾细胞数量中进一步观察到这种刺激作用，在用弗氏完全佐剂（FCA）或仅用FCA刺激抗原后，获得了正常值200%的细胞数量。CY对抗体形成的抑制作用可通过来自脾脏、淋巴结、派尔集合淋巴结或胸腺及骨髓的细胞恢复，表明CY处理导致B细胞和T细胞均存在缺陷。通过用CY处理小鼠的脾脏或淋巴结细胞重建无反应性小鼠，并添加或不添加正常骨髓和胸腺细胞，研究了CY处理的靶细胞。这表明虽然B细胞是CY作用的主要靶细胞，但对T细胞群体的影响也相当大。然而，当CY处理后进行抗原刺激时，添加骨髓细胞时淋巴结细胞的活性会急剧增加。这种现象类似于在有抗原存在的情况下，在接受致死性照射的小鼠脾脏中胸腺细胞的培育。
