Department of Chemical Engineering, University of Massachusetts Amherst, N533 Life Sciences Laboratories, 240 Thatcher Road, MA 01003-930, USA.
Department of Chemical Engineering, University of Massachusetts Amherst, N533 Life Sciences Laboratories, 240 Thatcher Road, MA 01003-930, USA.
Carbohydr Polym. 2014 Nov 26;113:561-8. doi: 10.1016/j.carbpol.2014.06.075. Epub 2014 Jul 31.
Due to the persistent spread of antibiotic resistance, commercial antibiotic treatments are proving ineffective. Cinnamaldehyde (CA), a volatile essential oil, eradicates pathogens non-specifically. However, the ability to incorporate essential oils into nanofiber mats has not yet been demonstrated, and, only six studies have electrospun two immiscible phases. Here, CA (0.5 and 5.0%) was incorporated into chitosan/poly(ethylene oxide) (PEO) solutions that were successfully electrospun into mats with ∼ 50 nm fiber diameters. Solid-state NMR results corroborated with release studies wherein the 5.0% CA mats released a statistically higher amount of CA-liquid (545% more) and CA-vapor (279% more) than the 0.5% CA mats. In time dependent cytotoxicity studies, the intrinsic antibacterial activity of chitosan along with the quick release of CA enabled high inactivation rates against Escherichia coli and Pseudomonas aeruginosa. For the first time we have demonstrated chitosan/CA/PEO nanofiber mats can serve as CA delivery vehicles that potentially eradicate pseudomonas infections.
由于抗生素耐药性的持续传播，商业抗生素治疗方法正在失效。肉桂醛（CA）是一种挥发性精油，可特异性地消除病原体。然而，将精油纳入纳米纤维垫的能力尚未得到证明，只有六项研究已经将两种不混溶的相电纺丝。在这里，CA（0.5%和 5.0%）被掺入壳聚糖/聚（氧化乙烯）（PEO）溶液中，这些溶液成功地电纺成具有约 50nm 纤维直径的垫子。固态 NMR 结果与释放研究相吻合，其中 5.0%CA 垫释放的 CA-液体（多 545%）和 CA-蒸汽（多 279%）的量明显高于 0.5%CA 垫。在时间依赖性细胞毒性研究中，壳聚糖的固有抗菌活性以及 CA 的快速释放使大肠杆菌和铜绿假单胞菌的失活率很高。我们首次证明壳聚糖/CA/PEO 纳米纤维垫可以作为 CA 输送载体，有可能根除铜绿假单胞菌感染。
