Advanced Materials Processing Group, Engineering Physics Study Program, Institut Teknologi Bandung, Bandung 40132, Indonesia.
Materials Science and Engineering Research Group, Faculty of Mechanical and Aerospace Engineering, Institut Teknologi Bandung, Bandung 40132, Indonesia.
Int J Nanomedicine. 2020 Aug 25;15:6433-6449. doi: 10.2147/IJN.S261483. eCollection 2020.
Electrospun nanofibers based on tuber (CET) protein are considered as a promising material for wound dressing applications. However, the use of these nanofibers in aqueous conditions has poor stability. The present study was performed to obtain insights into the crosslinked electrospun CET's protein-chitosan (CS)-poly(ethylene oxide) (PEO) nanofibers and to evaluate their potential for wound dressing applications.
The electrospun nanofibers were crosslinked with glutaraldehyde (GA) vapor and heat treatment (HT) to enhance their physicochemical stability. The crosslinked nanofibers were characterized by protein profiles, morphology structures, thermal behavior, mechanical properties, and degradation behavior. Furthermore, the antibacterial properties and cytocompatibility were analyzed by antibacterial assessment and cell proliferation.
The protein profiles of the electrospun CET's protein-CS-PEO nanofibers before and after HT crosslinking contained one major bioactive protein with a molecular weight of 14.4 kDa. Scanning electron microscopy images of the crosslinked nanofibers indicated preservation of the structure after immersion in phosphate buffered saline. The crosslinked nanofibers resulted in higher ultimate tensile strength and lower ultimate strain compared to the non-crosslinked nanofibers. GA vapor crosslinking showed higher water stability compared to HT crosslinking. The in vitro antibacterial activity of the crosslinked nanofibers showed a stronger bacteriostatic effect on than on . Human skin fibroblast cell proliferation on crosslinked GA vapor and HT nanofibers with 1% (w/v) CS and 2% (w/v) CET's protein demonstrated the highest among all the other crosslinked nanofibers after seven days of cell culture. Cell proliferation and cell morphology results revealed that introducing higher CET's protein concentration on crosslinked nanofibers could increase cell proliferation of the crosslinked nanofibers.
These results are promising for the potential use of the crosslinked electrospun CET's protein-CS-PEO nanofibers as bioactive wound dressing materials.
基于块茎（CET）蛋白的静电纺纳米纤维被认为是一种有前途的伤口敷料应用材料。然而，这些纳米纤维在水相条件下的使用稳定性较差。本研究旨在深入了解交联静电纺 CET 蛋白-壳聚糖（CS）-聚氧化乙烯（PEO）纳米纤维，并评估其在伤口敷料应用中的潜力。
通过戊二醛（GA）蒸气交联和热处理（HT）对电纺纳米纤维进行交联，以增强其物理化学稳定性。通过蛋白质图谱、形态结构、热行为、力学性能和降解行为对交联纳米纤维进行了表征。此外，通过抗菌评估和细胞增殖分析了抗菌性能和细胞相容性。
HT 交联前后电纺 CET 蛋白-CS-PEO 纳米纤维的蛋白质图谱均含有一种主要的生物活性蛋白，分子量为 14.4 kDa。交联纳米纤维的扫描电子显微镜图像表明，在浸入磷酸盐缓冲盐水后结构得以保留。与非交联纳米纤维相比，交联纳米纤维的最终拉伸强度更高，最终应变更低。与 HT 交联相比，GA 蒸气交联具有更高的水稳定性。交联纳米纤维的体外抗菌活性对 的抑菌效果强于对 的抑菌效果。在体外培养七天后，含 1%（w/v）CS 和 2%（w/v）CET 蛋白的交联 GA 蒸气和 HT 纳米纤维上的人皮肤成纤维细胞增殖率在所有交联纳米纤维中最高。细胞增殖和细胞形态学结果表明，在交联纳米纤维上引入更高浓度的 CET 蛋白可以增加交联纳米纤维的细胞增殖。
这些结果为交联静电纺 CET 蛋白-CS-PEO 纳米纤维作为生物活性伤口敷料材料的潜在应用提供了希望。
