Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, 81531-980 Curitiba, PR, Brazil; Universidade da Região de Joinville, CEP 89219-710 Joinville, SC, Brazil.
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, 81531-980 Curitiba, PR, Brazil.
Carbohydr Polym. 2014 Nov 26;113:588-96. doi: 10.1016/j.carbpol.2014.07.057. Epub 2014 Aug 2.
Glucans comprise an important class of polysaccharides present in basidiomycetes with potential biological activities. A (1 → 3)-β-D-glucan was isolated from Pleurotus sajor-caju via extraction with hot water followed by fractionation by freeze-thawing and finally by dimethyl sulfoxide extraction. The purified polysaccharide showed a (13)C-NMR spectrum with six signals consisting of a linear glucan with a β-anomeric signal at 102.8 ppm and a signal at 86.1 ppm relative to O-3 substitution. The other signals at 76.2, 72.9, 68.3, and 60.8 ppm were attributed to C5, C2, C4, and C6, respectively. This structure was confirmed by methylation analysis, and HSQC studies. The β-d-glucan from P. sajor-caju presented an immunomodulatory activity on THP-1 macrophages, inhibited the inflammatory phase of nociception induced by formalin in mice, and reduced the number of total leukocytes and myeloperoxidase levels induced by LPS. Taken together, these results demonstrate that this β-d-glucan exhibits a significant anti-inflammatory activity.
葡聚糖是担子菌中一类重要的多糖，具有潜在的生物活性。从糙皮侧耳中提取的（1→3）-β-D-葡聚糖，采用热水提取、反复冻融分级和二甲基亚砜提取的方法进行分离。纯化的多糖的 13C-NMR 谱显示出 6 个信号，由线性葡聚糖组成，β-端基异头碳信号位于 102.8 ppm，相对于 O-3 取代的信号位于 86.1 ppm。其他信号位于 76.2、72.9、68.3 和 60.8 ppm，分别归因于 C5、C2、C4 和 C6。该结构通过甲基化分析和 HSQC 研究得到证实。糙皮侧耳β-d-葡聚糖对 THP-1 巨噬细胞具有免疫调节活性，抑制甲醛诱导的小鼠疼痛感受的炎症期，并降低 LPS 诱导的总白细胞数和髓过氧化物酶水平。综上所述，这些结果表明，这种β-d-葡聚糖具有显著的抗炎活性。
