硝酸盐诱导的大鼠肝脏生化及组织病理学变化：希氏画眉草的改善作用
Nitrate-induced biochemical and histopathological changes in the liver of rats: ameliorative effect of Hyparrhenia hirta.
作者信息
Bouaziz-Ketata Hanen, Salah Ghada Ben, Salah Hichem Ben, Marrekchi Rim, Jamoussi Kamel, Boudawara Tahia, Fakhfekh Faiza, Zeghal Najiba
机构信息
Animal Physiology Laboratory, UR/11 ES70, Sfax Faculty of Sciences, University of Sfax, BP1171, Sfax 3000, Tunisia.
Laboratory of Human Molecular Genetics, Faculty of Medicine, Sfax 3029, Tunisia.
出版信息
Biomed Environ Sci. 2014 Sep;27(9):695-706. doi: 10.3967/bes2014.105.
OBJECTIVE
The present study investigated the protective role of Hyparrhenia hirta (H. hirta) against sodium nitrate (NaNO3)-induced hepatoxicity.
METHODS
Male Wistar rats were randomly divided into three groups: a control group and two treated groups during 50 d with NaNO3 administered either alone in drinking water or co-administered with H. hirta.
RESULTS
NaNO3 treatment induced a significant increase in serum levels of glucose, total cholesterol and triglyceride while serum total protein level decreased significantly. Transaminases and lactate deshydrogenase activities in serum were elevated indicating hepatic cells' damage after treatment with NaNO3. The hyperbilirubinemia and the increased serum gamma glutamyl transferase activities suggested the presence of cholestasis in NaNO3 exposed rats. In parallel, a significant increase in malondialdehyde level along with a concomitant decrease in total glutathione content and superoxide dismutase, catalase and glutathione peroxidase activities were observed in the liver after NaNO3 treatment. Furthermore, nitrate caused a significant induction of DNA fragmentation. These modifications in NaNO3-treated rats corresponded histologically with hepatocellular necrosis and mononuclear cells infiltration. H. hirta supplementation showed a remarkable amelioration of the abnormalities cited above.
CONCLUSION
The results concluded that the treatment with H. hirta had a significant role in protecting the animals from nitrate-induced liver dysfunction.
目的
本研究调查了牛鞭草对硝酸钠（NaNO₃）诱导的肝毒性的保护作用。
方法
雄性Wistar大鼠随机分为三组：对照组和两个处理组，在50天内，一组单独饮用含NaNO₃的水，另一组饮用含NaNO₃并同时添加牛鞭草的水。
结果
NaNO₃处理导致血清葡萄糖、总胆固醇和甘油三酯水平显著升高，而血清总蛋白水平显著降低。血清中转氨酶和乳酸脱氢酶活性升高，表明用NaNO₃处理后肝细胞受到损伤。高胆红素血症和血清γ-谷氨酰转移酶活性增加表明NaNO₃暴露大鼠存在胆汁淤积。同时，NaNO₃处理后肝脏中丙二醛水平显著升高，总谷胱甘肽含量以及超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性随之降低。此外，硝酸盐导致DNA片段化显著增加。NaNO₃处理大鼠的这些变化在组织学上与肝细胞坏死和单核细胞浸润相对应。添加牛鞭草显示出对上述异常情况的显著改善。
结论
结果表明，用牛鞭草处理对保护动物免受硝酸盐诱导的肝功能障碍具有重要作用。
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