Laboratory of Biochemistry and Environmental Toxicology, Department of Biochemistry, Faculty of Sciences, University Badji Mokhtar-Annaba, 23000, Annaba, Algeria.
Laboratory of Animal Ecophysiology, Department of Biology, Faculty of Sciences, University Badji Mokhtar-Annaba, 23000, Annaba, Algeria.
Afr Health Sci. 2020 Mar;20(1):413-425. doi: 10.4314/ahs.v20i1.48.
Nitrate (NO3) is the most common chemical contaminant in the world's ground water aquifer. Oxidative stress has been proposed as a possible mechanism involved in NO3 toxicity on non-target organism.
The current study aimed to elucidate the potential protective effect of (pumpkin seed oil, PSO) against hepatotoxicity induced by sodium nitrate.
Wistar rats were exposed either to NaNO (200 mg/kg bw) in drinking water in drinking water, or to 4ml PSO/kg bw by gavage or to their combination. Oxidative stress parameters, biochemical biomarkers and liver histopathological examination were determined.
Our data showed that the exposure of rats to NaNO caused significant changes of some haematological parameters compared to the control. In addition, there was a significant elevation of the levels of biochemical markers as that of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and lactate dehydrogenase when compared with the control. Furthermore, exposure of rats to NaNO induced liver oxidative stress as indicated by the increase of malondialdehyde, progressive oxidation of protein products and protein carbonyl levels. In addition, a reduction in anti-oxidant status (catalase, glutathione peroxidase, glutathione-S-transferase and superoxide dismutase, reduced glutathione and vitamin C) was observed.
Co-administration of PSO to the NaNO restored most parameters cited above to near-normal values. Therefore, the present investigation revealed the ability of PSO to attenuate NaNO-induced oxidative damage.
硝酸盐（NO3）是世界地下含水层中最常见的化学污染物。氧化应激已被提出是硝酸盐对非靶标生物毒性的一种可能机制。
本研究旨在阐明南瓜籽油（PSO）对亚硝酸钠诱导的肝毒性的潜在保护作用。
Wistar 大鼠分别通过饮用水（200mg/kg bw 的 NaNO）、灌胃（4ml/kg bw 的 PSO）或两者联合暴露。测定氧化应激参数、生化生物标志物和肝组织病理学检查。
我们的数据表明，与对照组相比，NaNO 暴露使大鼠的一些血液学参数发生了显著变化。此外，与对照组相比，天冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶和乳酸脱氢酶的生化标志物水平显著升高。此外，NaNO 的暴露诱导了肝脏氧化应激，表现为丙二醛增加、蛋白质产物和蛋白质羰基水平的渐进氧化。此外，抗氧化状态（过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽-S-转移酶、超氧化物歧化酶、还原型谷胱甘肽和维生素 C）下降。
PSO 与 NaNO 联合给药可使上述大多数参数恢复到接近正常水平。因此，本研究揭示了 PSO 减轻 NaNO 诱导的氧化损伤的能力。
