Gianaroli Luca, Magli M Cristina, Pomante Alessandra, Crivello Anna M, Cafueri Giulia, Valerio Marzia, Ferraretti Anna P
Reproductive Medicine Unit, Società Italiana Studi di Medicina della Riproduzione, Bologna, Italy.
Reproductive Medicine Unit, Società Italiana Studi di Medicina della Riproduzione, Bologna, Italy.
Fertil Steril. 2014 Dec;102(6):1692-9.e6. doi: 10.1016/j.fertnstert.2014.08.021. Epub 2014 Sep 23.
To investigate the presence of DNA in blastocyst fluids (BFs) and to estimate whether the chromosomal status predicted by its analysis corresponds with the ploidy condition in trophectoderm (TE) cells, the whole embryo, and that predicted by polar bodies (PBs) or blastomeres.
Prospective study.
In vitro fertilization unit.
PATIENT(S): Seventeen couples undergoing preimplantation genetic screening with the use of array comparative genomic hybridization on PBs (n = 12) or blastomeres (n = 5).
INTERVENTION(S): BFs and TE cells were retrieved from 51 blastocysts for separate chromosomal analysis.
MAIN OUTCOME MEASURE(S): Presence of DNA in BFs and assessment of the corresponding chromosome condition; correlation with the results in TE cells and those predicted by the analysis done at earlier stages.
RESULT(S): DNA was detected in 39 BFs (76.5%). In 38 of 39 cases (97.4%) the ploidy condition of BFs was confirmed in TE cells, and the rate of concordance per single chromosome was 96.6% (904/936). In relation to the whole embryo, the ploidy condition corresponded in all cases with a per-chromosome concordance of 98.1%. The testing of PBs and blastomeres had 93.3% and 100% prediction of BF ploidy condition with a concordance per chromosome of 93.5% and 94%, respectively.
CONCLUSION(S): Blastocentesis could represent an alternative source of material for chromosomal testing, because the BF is highly predictive of the embryo ploidy condition and chromosome content. Our data confirm the relevance of the oocyte and of the early-cleavage embryo in determining the ploidy condition of the resulting blastocyst.
研究囊胚液（BF）中DNA的存在情况，并评估通过其分析预测的染色体状态是否与滋养外胚层（TE）细胞、整个胚胎以及通过极体（PB）或卵裂球预测的倍性状况相符。
前瞻性研究。
体外受精单位。
17对接受植入前基因筛查的夫妇，使用PB（n = 12）或卵裂球（n = 5）的阵列比较基因组杂交技术。
从51个囊胚中获取BF和TE细胞进行单独的染色体分析。
BF中DNA的存在情况以及相应染色体状况的评估；与TE细胞结果以及早期阶段分析预测结果的相关性。
在39份BF中检测到DNA（76.5%）。在39例中的38例（97.4%）中，BF的倍性状况在TE细胞中得到证实，单条染色体的一致率为96.6%（904/936）。相对于整个胚胎，倍性状况在所有病例中均相符，每条染色体的一致率为98.1%。PB和卵裂球检测对BF倍性状况的预测率分别为93.3%和100%，每条染色体的一致率分别为93.5%和94%。
囊胚穿刺术可作为染色体检测的另一种材料来源，因为BF对胚胎倍性状况和染色体含量具有高度预测性。我们的数据证实了卵母细胞和早期卵裂胚胎在确定所得囊胚倍性状况方面的相关性。
