多发性硬化症患者T细胞受体β链基因的种系库

The germline repertoire of T-cell receptor beta-chain genes in patients with multiple sclerosis.

作者信息

Biddison W E, Beall S S, Concannon P, Charmley P, Gatti R A, Hood L E, McFarland H F, McFarlin D E

机构信息

Neuroimmunology Branch, National Institute of Neurological, Communicative Disorders, and Stroke, Bethesda, MD 20892.

出版信息

Res Immunol. 1989 Feb;140(2):212-5; discussion 245-8. doi: 10.1016/0923-2494(89)90089-8.

DOI:10.1016/0923-2494(89)90089-8

PMID:2525795

Abstract

The T-cell receptor (TCR) beta-chain gene repertoire of 40 multiple sclerosis (MS) patients was compared to that of 100 normal individuals. V-beta probes that represent 14 different V-beta subfamilies plus a C-beta probe were used to identify 53 separate beta-chain gene segments. No duplication or deletion of any of these 53 gene segments was found in the MS patients. Restriction fragment length polymorphism (RFLP) alleles detected by V-beta 8, V-beta 11 and C-beta probes defined 8 different beta-chain haplotypes. The distribution of these haplotypes in Caucasian MS patients and normal individuals was significantly different (p = 0.012). Comparison of the DR2+ subset of MS patients (n = 32) to a second group of 43 Caucasian DR2+ normal individuals revealed that the distribution of these beta-chain haplotypes was significantly different in these two populations (p = 0.015). These results suggest that an MS susceptibility gene(s) may be located in the region of the TCR/beta-chain gene complex.

摘要

将40例多发性硬化症（MS）患者的T细胞受体（TCR）β链基因库与100名正常个体的进行了比较。使用代表14个不同V-β亚家族的V-β探针以及一个C-β探针来鉴定53个不同的β链基因片段。在MS患者中未发现这53个基因片段中的任何一个存在重复或缺失。由V-β8、V-β11和C-β探针检测到的限制性片段长度多态性（RFLP）等位基因定义了8种不同的β链单倍型。这些单倍型在白种人MS患者和正常个体中的分布存在显著差异（p = 0.012）。将MS患者的DR2 +亚组（n = 32）与另一组43名白种人DR2 +正常个体进行比较，结果显示这两个群体中这些β链单倍型的分布存在显著差异（p = 0.015）。这些结果表明，MS易感基因可能位于TCR/β链基因复合体区域。