Key Laboratory of Luminescence and Real-Time, Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715 (P.R. China), Fax: (+86) 23-68251048.
Chemistry. 2014 Nov 3;20(45):14642-9. doi: 10.1002/chem.201403699. Epub 2014 Sep 26.
Sensitive and rapid detection of multiple analytes and the collection of components from complex samples are important in fields ranging from bioassays/chemical assays, clinical diagnosis, to environmental monitoring. A convenient strategy for creating magnetically encoded luminescent CdTe@SiO2 @n Fe3 O4 composite nanoparticles, by using a layer-by-layer self-assembly approach based on electrostatic interactions, is described. Silica-coated CdTe quantum dots (CdTe@SiO2 ) serve as core templates for the deposition of alternating layers of Fe3 O4 magnetic nanoparticles and poly(dimethyldiallyl ammonium chloride), to construct CdTe@SiO2 @n Fe3 O4 (n=1, 2, 3, …︁) composite nanoparticles with a defined number (n) of Fe3 O4 layers. Composite nanoparticles were characterized by zeta-potential analysis, fluorescence spectroscopy, vibrating sample magnetometry, and transmission electron microscopy, which showed that the CdTe@SiO2 @n Fe3 O4 composite nanoparticles exhibited excellent luminescence properties coupled with well-defined magnetic responses. To demonstrate the utility of these magnetically encoded nanoparticles for near-simultaneous detection and separation of multiple components from complex samples, three different fluorescently labeled IgG proteins, as model targets, were identified and collected from a mixture by using the CdTe@SiO2 @n Fe3 O4 nanoparticles.
敏感且快速地检测多种分析物并从复杂样品中收集组分在生物测定/化学分析、临床诊断到环境监测等领域都非常重要。本研究通过使用基于静电相互作用的层层自组装方法，描述了一种简便的策略，用于创建具有磁性编码的发光 CdTe@SiO2@nFe3O4 复合纳米粒子。涂覆有二氧化硅的碲化镉量子点（CdTe@SiO2）用作沉积交替层的铁磁纳米颗粒和聚（二甲基二烯丙基氯化铵）的核模板，以构建具有定义数量（n）的 Fe3O4 层的 CdTe@SiO2@nFe3O4（n=1、2、3、……）复合纳米粒子。通过 ζ 电位分析、荧光光谱、振动样品磁强计和透射电子显微镜对复合纳米粒子进行了表征，结果表明，CdTe@SiO2@nFe3O4 复合纳米粒子具有优异的发光性能和明确的磁响应。为了证明这些磁性编码纳米粒子在从复杂样品中同时检测和分离多种成分方面的实用性，使用 CdTe@SiO2@nFe3O4 纳米粒子从混合物中鉴定并收集了三种不同的荧光标记 IgG 蛋白作为模型靶标。
