Yang Guobin, Li Xiaoyan, Yuan Guohua, Liu Pingxian, Fan Mingwen
The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan.
The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan; Department of Endodontics, School and Hospital of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Shandong, China.
J Endod. 2014 Nov;40(11):1771-7. doi: 10.1016/j.joen.2014.04.012. Epub 2014 Sep 23.
Dental papilla cells (DPCs) are precursors of odontoblasts and have the potential to differentiate into odontoblasts. Osteoblasts and odontoblasts have many common characteristics. Osterix (Osx) is essential for osteoblast differentiation. However, no information is available for the effects of Osx on the odontoblastic differentiation of DPCs. The purpose of this study was to investigate the effects of Osx on the proliferation and odontoblastic differentiation of DPCs.
An immortalized human dental papilla cell (hDPC) line was used. Osx was stably overexpressed or knocked down in hDPCs with infection of lentiviral particles to determine its biological effects on hDPCs. The proliferation of cells was measured by the 5-ethynyl-2'-deoxyuridine incorporation assay and direct cell counting. Expressions of dentin sialophosphoprotein, nestin, dentin matrix protein 1, and alkaline phosphatase were detected by real-time polymerase chain reaction to determine the odontoblastic differentiation of cells. The mineralization ability of cells was evaluated by von Kossa staining and alkaline phosphatase activity assay.
Overexpression of Osx retarded the proliferation of hDPCs, whereas knockdown of Osx increased the cell proliferation. Overexpression of Osx promoted the odontoblastic differentiation of hDPCs by up-regulating odontoblastic differentiation genes and increased the mineralization ability of hDPCs. Knockdown of Osx down-regulated odontoblastic differentiation genes and decreased the mineralization ability of hDPCs.
Osx might function as a potential regulator for the proliferation and odontoblastic differentiation of hDPCs.
牙乳头细胞（DPCs）是成牙本质细胞的前体细胞，具有分化为成牙本质细胞的潜力。成骨细胞和成牙本质细胞有许多共同特征。osterix（Osx）对成骨细胞分化至关重要。然而，关于Osx对DPCs向成牙本质细胞分化的影响尚无相关信息。本研究的目的是探讨Osx对DPCs增殖和成牙本质细胞分化的影响。
使用永生化的人牙乳头细胞（hDPC）系。通过慢病毒颗粒感染在hDPCs中稳定过表达或敲低Osx，以确定其对hDPCs的生物学效应。通过5-乙炔基-2'-脱氧尿苷掺入试验和直接细胞计数来测量细胞增殖。通过实时聚合酶链反应检测牙本质涎磷蛋白、巢蛋白、牙本质基质蛋白1和碱性磷酸酶的表达，以确定细胞的成牙本质细胞分化。通过冯科萨染色和碱性磷酸酶活性测定评估细胞的矿化能力。
Osx的过表达抑制了hDPCs的增殖，而Osx的敲低则增加了细胞增殖。Osx的过表达通过上调成牙本质细胞分化基因促进了hDPCs的成牙本质细胞分化，并增加了hDPCs的矿化能力。Osx的敲低下调成牙本质细胞分化基因并降低了hDPCs的矿化能力。
Osx可能是hDPCs增殖和成牙本质细胞分化的潜在调节因子。
