同位素对NAD⁺与乳酸脱氢酶结合的影响。
Isotope effects on binding of NAD+ to lactate dehydrogenase.
作者信息
LaReau R D, Wan W, Anderson V E
机构信息
Department of Chemistry, Brown University, Providence, Rhode Island 02912.
出版信息
Biochemistry. 1989 Apr 18;28(8):3619-24. doi: 10.1021/bi00434a070.
PMID:2525926
Abstract
The isotope effect on binding [4-2H]NAD+ and [4-3H]NAD+ to lactate dehydrogenase has been shown to be 1.10 +/- 0.03 by whole molecule isotope ratio mass spectrometry and 1.085 +/- 0.01 by 3H/14C scintillation counting. These values demonstrate that specific interactions of the nicotinamide ring with the enzyme make the C-H bond at C-4 less stiff in the binary complex.
摘要
通过全分子同位素比率质谱法显示，[4-2H]NAD+和[4-3H]NAD+与乳酸脱氢酶结合的同位素效应为1.10±0.03，通过3H/14C闪烁计数法得到的值为1.085±0.01。这些值表明，烟酰胺环与酶的特异性相互作用使二元复合物中C-4处的C-H键刚性降低。
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