Zhu Xin, Yi Tan, Chen Tao, Bin Shi-Yu
a College of Veterinary Medicine, Hunan Agriculture University , Changsha , Hunan , P.R. China and.
b College of Life Sciences, Guangxi Normal University , Guilin , Guangxi , P.R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1777-8. doi: 10.3109/19401736.2014.963808. Epub 2014 Sep 26.
In this paper, the complete mitochondrial genome of the hybrid of Oreochromis niloticus (♀) × Oreochromis aureus (♂) was determined using PCR-based method. The mitogenome was 16,663 bp in length, containing the same gene order and an identical number of genes or regions with the other Cichlid fishes, including 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 putative control region. The overall composition of the mitogenome was 30.92% C, 27.98% A, 25.54% T, 15.56% G, with a slight AT bias of 53.52% occurs in the hybrid mitogenome. All the protein-coding genes were initiated by typical ATG codon, except for COX1 gene with the initiation codon GTG. Eight genes end with the complete stop codon TAA or TAG, while the COX2, COX3, ND3, ND4 and Cytb genes terminated with an incomplete stop codon T. The complete mitochondrial genome of Oreochromis niloticus (♀) × Oreochromis aureus (♂) may provide important DNA molecular data for further elucidation of evolutionary mechanisms in the hybrid fish of Cichlidae.
本文采用基于PCR的方法测定了尼罗罗非鱼（♀）×奥利亚罗非鱼（♂）杂交种的完整线粒体基因组。该线粒体基因组长度为16,663 bp，基因排列顺序以及基因或区域数量与其他丽鱼科鱼类相同，包括13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个假定的控制区。线粒体基因组的总体组成为：C占30.92%，A占27.98%，T占25.54%，G占15.56%，杂交线粒体基因组存在轻微的AT偏向性，AT含量为53.52%。除COX1基因起始密码子为GTG外，所有蛋白质编码基因均由典型的ATG密码子起始。8个基因以完整的终止密码子TAA或TAG结束，而COX2、COX3、ND3、ND4和Cytb基因以不完整的终止密码子T结束。尼罗罗非鱼（♀）×奥利亚罗非鱼（♂）的完整线粒体基因组可能为进一步阐明丽鱼科杂交鱼的进化机制提供重要的DNA分子数据。
