Jang Yo-Soon, Oh Sung-Yong, Lee Eun Kyung, Myoung Jung-Goo, Park Jeong-Ho, Kim Ki-Yong, Kim Sung
a East Sea Environment Research Division , Korea Institute of Ocean Science & Technology , Hujung-ri, Jukbyeon-myun, Uljin-gun , Gyeongbuk , Republic of Korea .
b Department of Convergence Study on the Ocean Science and Technology , Ocean Science and Technology School, Korea Maritime and Ocean University , Dongsamg-dong, Yeongdo-gu , Busan , Republic of Korea .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1239-40. doi: 10.3109/19401736.2014.945536. Epub 2014 Aug 7.
Sebastes vulpes is considered a valid species despite the presence of extensive hybrids. To obtain the basic genetic intraspecific structure, we analyzed the complete mitogenome of S. vulpes using next-generation sequencing. The complete mitogenome was 16,462 bp in length containing 13 protein-coding genes (PCGs), 2 rRNAs, 22 tRNAs and 1 control region. The complete mitogenome comprised 27.7% A, 26.5% T, 17.1% G and 28.6% C, showing a minor AT bias (54.3%). The initial codon in all PCGs was ATG, excluding COX1 (GTG). The majority of stop codons in the PCGs were TAA, excluding ND1 and ND3 (TAG), ND4 (AGA) and Cytb (incomplete termination codon, T). All tRNAs had a typical cloverleaf structure, excluding tRNA(Ser (AGY)), which lacked the DHU arm. The complete mitogenome of S. vulpes can be used to study hybridisation and on-going speciation.
尽管存在大量杂交种,但狐形平鲉仍被视为一个有效物种。为了获得基本的种内遗传结构,我们使用下一代测序技术分析了狐形平鲉的完整线粒体基因组。完整的线粒体基因组长度为16,462 bp,包含13个蛋白质编码基因(PCGs)、2个rRNA、22个tRNA和1个控制区。完整的线粒体基因组由27.7%的A、26.5%的T、17.1%的G和28.6%的C组成,显示出轻微的AT偏向(54.3%)。所有PCGs中的起始密码子均为ATG,但COX1除外(GTG)。PCGs中的大多数终止密码子为TAA,但ND1和ND3除外(TAG)、ND4(AGA)和Cytb(不完全终止密码子,T)。所有tRNA均具有典型的三叶草结构,但tRNA(Ser (AGY))除外,它缺少DHU臂。狐形平鲉的完整线粒体基因组可用于研究杂交和正在进行的物种形成。