通过线粒体膜中的多萜醇中间体研究N-糖蛋白生物合成。

Study of the N-glycoprotein biosynthesis through dolichol intermediates in the mitochondrial membranes.

Levrat C, Ardail D, Morelis R, Louisot P

Department of Biochemistry, University of Lyon, Lyon-Sud Medical School, INSERM-CNRS U.189, Oullins, France.

Int J Biochem. 1989;21(3):265-78. doi: 10.1016/0020-711x(89)90184-5.

Abstract

In the mitochondria, the biosynthesis of N-glycoprotein products, through the dolichol intermediates pathway, appears in the outer and in the inner membranes. 2. The biosynthesis of dolichol-pyrophosphoryl-N-acetyl-glucosamine, dolichol-pyrophosphoryl-di-N-acetylchitobiose, dolichol-phosphoryl-glucose and dolichol-phosphoryl-mannose is effective in both membranes. 3. The lipid-linked oligosaccharides biosynthesized in both membranes contain high mannose-type oligosaccharides ranging in size from Man9-GlcNac2 to Man4-GlcNac2. 4. The assembly of the dolichol-pyrophosphoryl-oligosaccharides on the trimannosidic core begins by the elongation of the alpha-1,3 mannose branch in the outer membrane and of the alpha-1,6 mannose branch in the inner membrane.

摘要

在线粒体中，通过多萜醇中间产物途径合成的N-糖蛋白产物出现在外膜和内膜中。2. 多萜醇焦磷酸-N-乙酰葡糖胺、多萜醇焦磷酸-二-N-乙酰壳二糖、多萜醇磷酸葡萄糖和多萜醇磷酸甘露糖的生物合成在这两层膜中均有效。3. 在这两层膜中生物合成的脂连接寡糖含有高甘露糖型寡糖，其大小范围从Man9-GlcNac2到Man4-GlcNac。（注：原文此处有误，应为Man9-GlcNAc2到Man5-GlcNAc2）4. 多萜醇焦磷酸寡糖在三甘露糖核心上的组装始于外膜中α-1,3甘露糖分支和内膜中α-1,6甘露糖分支的延伸。