Sakamoto Hiroaki, Matsushita Shonosuke, Hyodo Kazuyuki, Tokunaga Chiho, Sato Fujio, Hiramatsu Yuji
Department of Cardiovascular Surgery, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan
Faculty of Health Science, Tsukuba University of Technology, Tsukuba, Japan.
Acta Radiol. 2015 Sep;56(9):1105-7. doi: 10.1177/0284185114549570. Epub 2014 Sep 26.
Previously, in our laboratory, synchrotron radiation coronary microangiography (SRCA) using Langendorff-perfused rat hearts could visualize a coronary artery of 50 µm in diameter. However, in vivo rat SRCA poses the problem of compromised temporal resolution due to the rapid heart rate of rats.
To establish a simple method of in vivo rat SRCA with bradycardia induced by intravenous injection of adenosine triphosphate disodium hydrate (ATP).
SRCA was performed at the Photon Factory of the High Energy Accelerator Research Organization (Tsukuba, Japan). Eight male Wistar rats were anesthetized. A catheter for injecting the contrast material was inserted into the carotid artery. Temporary bradycardia was induced by an intravenous bolus injection of 5 mg of ATP, and SRCA was performed immediately thereafter.
After ATP administration, the average heart rate decreased from 388 to 73 beats per minute. As a result, we could detect a coronary artery as small as 45 µm in diameter.
Our SRCA system which has a high resolution of 9 µm per pixel could detect a coronary artery as small as 45 µm in diameter in the in vivo rat.
此前，在我们实验室，使用Langendorff灌注大鼠心脏的同步辐射冠状动脉微血管造影（SRCA）能够可视化直径为50微米的冠状动脉。然而，由于大鼠心率较快，活体大鼠SRCA存在时间分辨率受损的问题。
建立一种通过静脉注射三水合三磷酸腺苷二钠（ATP）诱导心动过缓的活体大鼠SRCA的简单方法。
SRCA在高能加速器研究组织的光子工厂（日本筑波）进行。8只雄性Wistar大鼠麻醉后，将用于注射造影剂的导管插入颈动脉。通过静脉推注5毫克ATP诱导暂时性心动过缓，随后立即进行SRCA。
给予ATP后，平均心率从每分钟388次降至73次。结果，我们能够检测到直径小至45微米的冠状动脉。
我们的SRCA系统每像素具有9微米的高分辨率，能够在活体大鼠中检测到直径小至45微米的冠状动脉。
