Sharon Shir, Salomon Eitan, Kranzler Chana, Lis Hagar, Lehmann Robert, Georg Jens, Zer Hagit, Hess Wolfgang R, Keren Nir
Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, Edmond J. Safra Campus, Givat Ram, The Hebrew University of Jerusalem, Jerusalem, Israel.
Institute for Theoretical Biology, Humboldt University Berlin, Invalidenstraße 43, D-10115 Berlin, Germany.
Biochim Biophys Acta. 2014 Dec;1837(12):1990-1997. doi: 10.1016/j.bbabio.2014.09.007.
Iron and manganese are part of a small group of transition metals required for photosynthetic electron transport. Here, we present evidence for a functional link between iron and manganese homeostasis. In the unicellular cyanobacterium, Synechocystis sp. PCC 6803, Fe and Mn deprivation resulted in distinct modifications of the physiological status. The effect on growth and photosynthetic activity under Fe limitation were more severe than those observed under Mn limitation. Moreover, the intracellular elemental quotas of Fe and Mn were found to be linked. Fe limitation reduced the intracellular Mn quota. Mn limitation did not exert a reciprocal effect on Fe quotas. Microarray analysis comparing Mn and Fe limitation revealed a stark difference in the extent of the transcriptional response to the two limiting conditions, reflective of the physiological responses. The effects of Fe limitation on the transcriptional network are widespread while the effects on Mn limitation are highly specific. Our analysis also revealed an overlap in the transcriptional response of specific Fe and Mn transporters. This overlap provides a framework for explaining Fe limitation induced changes in Mn quotas.
铁和锰是光合电子传递所需的一小部分过渡金属。在此，我们提供了铁和锰稳态之间功能联系的证据。在单细胞蓝藻集胞藻PCC 6803中，铁和锰缺乏导致生理状态的明显改变。铁限制条件下对生长和光合活性的影响比锰限制条件下观察到的更为严重。此外，发现铁和锰的细胞内元素配额是相关联的。铁限制降低了细胞内锰的配额。锰限制对铁配额没有产生相反的影响。比较锰限制和铁限制的微阵列分析揭示了对两种限制条件转录反应程度的显著差异，这反映了生理反应。铁限制对转录网络的影响广泛，而对锰限制的影响则高度特异。我们的分析还揭示了特定铁和锰转运蛋白转录反应的重叠。这种重叠为解释铁限制引起的锰配额变化提供了一个框架。
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