小儿高血压患者中内收蛋白GLY460TRP、血管紧张素转换酶I/D及血管紧张素原M235T的基因多态性
Gene polymorphisms of adducin GLY460TRP, ACE I/D, AND AGT M235T in pediatric hypertension patients.
作者信息
Kaplan İbrahim, Sancaktar Enver, Ece Aydın, Şen Velat, Tekkeşin Nilgün, Basarali Mustafa Kemal, Kelekci Selvi, Evliyaoglu Osman
机构信息
Department of Medical Biochemistry, Dicle University, Diyarbakir, Turkey.
Department of Medical Biochemistry, Cumhuriyet University, Sivas, Turkey.
出版信息
Med Sci Monit. 2014 Sep 28;20:1745-50. doi: 10.12659/MSM.892140.
BACKGROUND
Hypertension is a major global public health problem that affects both pediatric and adult populations. ACE I/D, AGT M235T, and ADD Gly460Trp polymorphisms are thought to be associated with primary hypertension. In the present study, we examined the frequency of these polymorphisms in a pediatric population with secondary hypertension.
MATERIAL AND METHODS
Included in the study were 58 hypertensive and 58 normotensive pediatric patients. ACE I/D and AGT M235T polymorphisms are determined by conventional PCR; ADD Gly460Trp polymorphism was investigated using PCR amplification of genomic DNA.
RESULTS
There were significant differences between the control group and pediatric hypertensive group in terms of ACE I/D (P<0.05) and AGT M235T (P<0.05) polymorphisms, but there were no differences in ADD Gly460Trp (P>0.05) polymorphism.
CONCLUSIONS
We suggest that RAS gene polymorphisms (ACE-I/D, AGT M235T) are significantly associated with susceptibility to diseases that lead to secondary hypertension.
背景
高血压是一个影响儿童和成人的主要全球公共卫生问题。血管紧张素转换酶（ACE）I/D、血管紧张素原（AGT）M235T和肾上腺髓质素（ADD）Gly460Trp基因多态性被认为与原发性高血压有关。在本研究中，我们检测了这些基因多态性在继发性高血压儿童人群中的频率。
材料与方法
本研究纳入了58例高血压儿童患者和58例血压正常的儿童患者。ACE I/D和AGT M235T基因多态性通过常规聚合酶链反应（PCR）测定；ADD Gly460Trp基因多态性通过基因组DNA的PCR扩增进行研究。
结果
对照组与儿童高血压组在ACE I/D（P<0.05）和AGT M235T（P<0.05）基因多态性方面存在显著差异，但在ADD Gly460Trp（P>0.05）基因多态性方面无差异。
结论
我们认为肾素-血管紧张素系统（RAS）基因多态性（ACE-I/D、AGT M235T）与导致继发性高血压的疾病易感性显著相关。
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