Yuan Ying, Dego Oudessa Kerro, Chen Xueyan, Abadin Eurife, Chan Shangfeng, Jory Lauren, Kovacevic Steven, Almeida Raul A, Oliver Stephen P
Zoetis, Veterinary Medicine Research and Development (VMRD), Global Therapeutic Research, 333 Portage Street, Kalamazoo, MI 49007.
Department of Animal Science, The University of Tennessee, Knoxville 37996.
J Dairy Sci. 2014 Dec;97(12):7668-73. doi: 10.3168/jds.2013-7637. Epub 2014 Sep 26.
The objective was to identify and sequence the sua gene (GenBank no. DQ232760; http://www.ncbi.nlm.nih.gov/genbank/) and detect Streptococcus uberis adhesion molecule (SUAM) expression by Western blot using serum from naturally S. uberis-infected cows in strains of S. uberis isolated in milk from cows with mastitis from geographically diverse areas of the world. All strains evaluated yielded a 4.4-kb sua-containing PCR fragment that was subsequently sequenced. Deduced SUAM AA sequences from those S. uberis strains evaluated shared >97% identity. The pepSUAM sequence located at the N terminus of SUAM was >99% identical among strains of S. uberis. Streptococcus uberis adhesion molecule expression was detected in all strains of S. uberis tested. These results suggest that sua is ubiquitous among strains of S. uberis isolated from diverse geographic locations and that SUAM is immunogenic.
目的是鉴定并对sua基因（GenBank编号：DQ232760；http://www.ncbi.nlm.nih.gov/genbank/）进行测序，并使用来自世界不同地理区域患乳房炎奶牛乳汁中分离的乳房链球菌菌株的、自然感染乳房链球菌奶牛的血清，通过蛋白质印迹法检测乳房链球菌粘附分子（SUAM）的表达。所有评估的菌株均产生了一个含4.4 kb sua的PCR片段，随后对其进行了测序。从所评估的那些乳房链球菌菌株推导的SUAM氨基酸序列具有>97%的同一性。位于SUAM N末端的pepSUAM序列在乳房链球菌菌株中>99%相同。在所有测试的乳房链球菌菌株中均检测到乳房链球菌粘附分子的表达。这些结果表明，sua在从不同地理位置分离的乳房链球菌菌株中普遍存在，并且SUAM具有免疫原性。
