Wang Linlin, Zheng Lingyun, Zhang Lei, Chen Ali, Qiu Cuihuan, Xu Jingfen, Yang Yongxia
ollege of Traditional Chinese Medicine, 2School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Aug;34(9):1301-4.
To study the metabolic changes of pancreatic extracts from insulin-resistant rats induced by fructose feeding using nuclear magnetic resonance ¹H spectroscopy (¹H NMR).
Sixteen Wistar rats were divided equally into control group and model group and given water and 10% fructose water for 8 weeks, respectively. The pancreatic tissues were then obtained for H NMR spectra analysis and principal component analysis (PCA).
Compared with the control rats, the rats in the model group showed significantly increased creatine, betaine/TMAO, taurine, glycine and myo-inositol and decreased levels of lipids, lactate, glutamate, choline and GPC/PC.
¹H NMR and pattern recognition can define the metabolic characteristics of the pancreatic tissue extracts from insulin-resistant rats and provide reliable metabolic evidence for studying the mechanisms of insulin resistance at the molecular level.
采用核磁共振¹H波谱法（¹H NMR）研究果糖喂养诱导的胰岛素抵抗大鼠胰腺提取物的代谢变化。
将16只Wistar大鼠平均分为对照组和模型组，分别给予水和10%果糖水，持续8周。然后获取胰腺组织进行¹H NMR光谱分析和主成分分析（PCA）。
与对照大鼠相比，模型组大鼠的肌酸、甜菜碱/氧化三甲胺、牛磺酸、甘氨酸和肌醇显著增加，脂质、乳酸、谷氨酸、胆碱和甘油磷酰胆碱/磷脂酰胆碱水平降低。
¹H NMR和模式识别可以确定胰岛素抵抗大鼠胰腺组织提取物的代谢特征，并为在分子水平研究胰岛素抵抗机制提供可靠的代谢证据。
