[果糖喂养诱导的胰岛素抵抗大鼠胰腺提取物的¹H NMR代谢组学研究]
[¹H NMR metabonomics study of pancreatic extracts from insulin-resistant rats induced by fructose feeding].
作者信息
Wang Linlin, Zheng Lingyun, Zhang Lei, Chen Ali, Qiu Cuihuan, Xu Jingfen, Yang Yongxia
机构信息
ollege of Traditional Chinese Medicine, 2School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China. E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Aug;34(9):1301-4.
OBJECTIVE
To study the metabolic changes of pancreatic extracts from insulin-resistant rats induced by fructose feeding using nuclear magnetic resonance ¹H spectroscopy (¹H NMR).
METHODS
Sixteen Wistar rats were divided equally into control group and model group and given water and 10% fructose water for 8 weeks, respectively. The pancreatic tissues were then obtained for H NMR spectra analysis and principal component analysis (PCA).
RESULTS
Compared with the control rats, the rats in the model group showed significantly increased creatine, betaine/TMAO, taurine, glycine and myo-inositol and decreased levels of lipids, lactate, glutamate, choline and GPC/PC.
CONCLUSION
¹H NMR and pattern recognition can define the metabolic characteristics of the pancreatic tissue extracts from insulin-resistant rats and provide reliable metabolic evidence for studying the mechanisms of insulin resistance at the molecular level.
目的
采用核磁共振¹H波谱法（¹H NMR）研究果糖喂养诱导的胰岛素抵抗大鼠胰腺提取物的代谢变化。
方法
将16只Wistar大鼠平均分为对照组和模型组，分别给予水和10%果糖水，持续8周。然后获取胰腺组织进行¹H NMR光谱分析和主成分分析（PCA）。
结果
与对照大鼠相比，模型组大鼠的肌酸、甜菜碱/氧化三甲胺、牛磺酸、甘氨酸和肌醇显著增加，脂质、乳酸、谷氨酸、胆碱和甘油磷酰胆碱/磷脂酰胆碱水平降低。
结论
¹H NMR和模式识别可以确定胰岛素抵抗大鼠胰腺组织提取物的代谢特征，并为在分子水平研究胰岛素抵抗机制提供可靠的代谢证据。
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