Zheng Hui-Ting, Li Ke-Ning, Chen Chi, Lin Wei-Dong, Wang Shu-Mei
Guangdong Pharmaceutical University, Guangzhou 510006, China.
Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica, State Administration of Traditional Chinese Medicine, Guangzhou 510006, China.
Zhongguo Zhong Yao Za Zhi. 2018 Jul;43(14):3012-3017. doi: 10.19540/j.cnki.cjcmm.20180327.003.
Pancreas metabonomic profiles of the type 2 diabetic rats' induced by streptozotocin(STZ) and high-sugar, high fat diet on the treatment of Renshenjian decoction(RSJD) after 8 weeks were investigated.In this study, 48 Rats were randomly divided into four groups: normal control (NC), Pathological model (PM), Renshenjian decoction(RSJD 3.76 g·kg⁻¹) and glimepiride control (GC 0.04 mg·kg⁻¹). They are induced insulin resistance model of type 2 diabetes mellitus by streptozotocin(STZ) after 4 weeks' high-sugar, high fat diet except for NC. After sucessful modeling, they are given intragastric administration respectively with same amount of saline, RSJD and glimepiride in 4 weeks. At the end of the 8th week, the pancreatic tissue of rats in each group was collected, and the ¹H-NMR spectrum was collected after being treated by certain method, and analyzed by principal component analysis (PCA). Compared with NC's rats, we found PM's a significant elevation in the level of leucine/isoleucine, valine, lactic acid, creatine but reduction in the level of inose and less obvious changes in the level of creatine, cholic acid, taurine in pancreatic extract. After having been recieved RSJD, reduction level in leucine/isoleucine, valine, alanine, creatine, choline, taurine are also found in pancreatic extract of RSJD's rats, together with the increase of creatinine and tryptophan levels. The results showed that RSJD could regulate the level of amino acids in pancreas of IR rats, promoting a recovery in the process of metabolism. It's helpful to simulate the metabolic changes of IR rats via ¹H-NMR for a further understanding to study the mechanism how RSJD treat IR rats.
研究链脲佐菌素（STZ）联合高糖高脂饮食诱导的2型糖尿病大鼠经人参煎剂（RSJD）治疗8周后的胰腺代谢组学特征。本研究将48只大鼠随机分为四组：正常对照组（NC）、病理模型组（PM）、人参煎剂组（RSJD 3.76 g·kg⁻¹）和格列美脲对照组（GC 0.04 mg·kg⁻¹）。除NC组外，其余大鼠经4周高糖高脂饮食后，用链脲佐菌素（STZ）诱导2型糖尿病胰岛素抵抗模型。造模成功后，4周内分别给予等量生理盐水、RSJD和格列美脲灌胃。在第8周结束时，收集每组大鼠的胰腺组织，经一定方法处理后采集¹H-NMR谱，并通过主成分分析（PCA）进行分析。与NC组大鼠相比，发现PM组大鼠胰腺提取物中亮氨酸/异亮氨酸、缬氨酸、乳酸、肌酸水平显著升高，而肌醇水平降低，肌酸、胆酸、牛磺酸水平变化不明显。给予RSJD后，RSJD组大鼠胰腺提取物中亮氨酸/异亮氨酸、缬氨酸、丙氨酸、肌酸、胆碱、牛磺酸水平也降低，同时肌酐和色氨酸水平升高。结果表明，RSJD可调节IR大鼠胰腺中氨基酸水平，促进代谢过程恢复。通过¹H-NMR模拟IR大鼠的代谢变化，有助于进一步深入了解RSJD治疗IR大鼠的机制。
