Hu Chen, Zhang Shiqing, Jin Xiaoyan, Tong Li
School of Chinese Medicine, Southern Medical University, Guangzhou 510515, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Aug;34(9):1365-9.
To study the effects of Meilian Xiaoke capsule (a traditional Chinese medicinal preparation) combined with metformin for protecting islet cells and lowering blood glucose in diabetic rats.
Rat models with type 2 diabetes, established by high-fat and high-glucose diet combined with streptozotocin (STZ) injection, were treated with a low dose of metformin, Meilian Xiaoke capsule, or both for 4 weeks by gavage. Blood glucose level was tested in the rats, and islet pathologies and changes in islet β cell number after the treatment were observed with HE staining and aldehyde fuchsin staining, respectively.
Treatment with metformin or Meilian Xiaoke capsule alone for 2 or 4 weeks did not produce significant improvement of blood glucose in the diabetic rats. Their combined treatment for 4 weeks resulted in significantly lowered blood glucose level and improved glucose tolerance with also obviously increased islet β cell number and lessened islet pathologies.
Meilian Xiaoke capsule and metformin show a synergistic effect to significantly enhance the therapeutic effect in rats with type 2 diabetes.
研究美连消渴胶囊（一种中药制剂）联合二甲双胍对糖尿病大鼠胰岛细胞的保护作用及降血糖作用。
采用高脂高糖饮食联合链脲佐菌素（STZ）注射建立2型糖尿病大鼠模型，通过灌胃给予低剂量二甲双胍、美连消渴胶囊或两者联合用药4周。检测大鼠血糖水平，分别用苏木精-伊红（HE）染色和醛复红染色观察治疗后胰岛病理变化及胰岛β细胞数量变化。
单独使用二甲双胍或美连消渴胶囊治疗2周或4周，糖尿病大鼠血糖无明显改善。两者联合治疗4周可显著降低血糖水平，改善糖耐量，同时胰岛β细胞数量明显增加，胰岛病理变化减轻。
美连消渴胶囊与二甲双胍具有协同作用，可显著增强对2型糖尿病大鼠的治疗效果。
