Saadati Hakimeh, Sheibani Vahid, Esmaeili-Mahani Saeed, Hajali Vahid, Mazhari Shahrzad
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran; Department of Physiology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Brain Res Bull. 2014 Sep;108:100-5. doi: 10.1016/j.brainresbull.2014.09.009. Epub 2014 Sep 28.
Previous studies have indicated that physical exercise plays a preventive role in synaptic plasticity deficits in the hippocampus of sleep-deprived male rats. The objective of the present study was to evaluate the effects of treadmill running on early long term potentiation (E-LTP) at the Cornu Ammonis (CA1) area of the hippocampus in sleep-deprived female rats. Intact and ovariectomiezed (OVX) female Wistar rats were used in the present study. The exercise protocol was four weeks treadmill running and the multiple platform method was applied to induce 72 h sleep deprivation (SD). We examine the effect of exercise and/or SD on synaptic plasticity using in vivo extracellular recording in the CA1 area of the hippocampus. The field excitatory post-synaptic potential (fEPSP) slope was measured before and 2h after high frequency stimulation (HFS) in the experimental groups. Field potential recording indicated that the induction and maintenance phase of E-LTP impaired in the sleep deprived animals compared to the other groups. After 72 h SD, E-LTP impairments were prevented by 4 weeks of regular treadmill exercise. In conclusion, the synaptic plasticity deficit in sleep-deprived female rats was improved by regular physical exercise. Further studies are suggested to evaluate the possible underlying mechanisms.
先前的研究表明，体育锻炼对睡眠剥夺的雄性大鼠海马体突触可塑性缺陷具有预防作用。本研究的目的是评估跑步机跑步对睡眠剥夺的雌性大鼠海马体角回（CA1）区早期长时程增强（E-LTP）的影响。本研究使用了完整和去卵巢（OVX）的雌性Wistar大鼠。运动方案为四周跑步机跑步，并采用多平台方法诱导72小时睡眠剥夺（SD）。我们使用海马体CA1区的体内细胞外记录来研究运动和/或睡眠剥夺对突触可塑性的影响。在实验组中，在高频刺激（HFS）前和后2小时测量场兴奋性突触后电位（fEPSP）斜率。场电位记录表明，与其他组相比，睡眠剥夺动物中E-LTP的诱导和维持阶段受损。在72小时睡眠剥夺后，四周的定期跑步机运动可预防E-LTP损伤。总之，定期体育锻炼改善了睡眠剥夺雌性大鼠的突触可塑性缺陷。建议进一步研究以评估可能的潜在机制。
