三种番茄环斑病毒分离株的全基因组序列：重配和重组的证据
Complete genome sequence of three tomato ringspot virus isolates: evidence for reassortment and recombination.
作者信息
Walker Melanie, Chisholm Joan, Wei Ting, Ghoshal Basudev, Saeed Hanna, Rott Michael, Sanfaçon Hélène
机构信息
Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, 4200 Highway 97, PO Box 5000, Summerland, BC, V0H 1Z0, Canada.
出版信息
Arch Virol. 2015 Feb;160(2):543-7. doi: 10.1007/s00705-014-2240-y. Epub 2014 Oct 1.
The genome sequence of tomato ringspot virus (ToRSV, a subgroup C nepovirus) is currently available for one raspberry isolate. In this study, we describe the complete genome sequence of three additional isolates from raspberry (Rasp1-2014), grapevine (GYV-2014) and prunus (13C280). The degree of nucleotide sequence identity shared between RNA1 and RNA2 in the 5'-terminal 900 nucleotides and 3' untranslated region varied from 98-99 % (13C280, GYV-2014) to 80 % (Rasp1-2014). Phylogenetic studies revealed distinct origins for Rasp1-2014 RNA1 and RNA2, suggesting reassortment. Two recombination events were also identified in the 3' UTR and 5'-terminal region of RNA1.
番茄环斑病毒（ToRSV，C组线虫传多面体病毒亚组）的基因组序列目前已有一份来自树莓的分离株。在本研究中，我们描述了另外三份分离株的完整基因组序列，分别来自树莓（Rasp1 - 2014）、葡萄（GYV - 2014）和李属（13C280）。RNA1和RNA2在5'端900个核苷酸和3'非翻译区之间共享的核苷酸序列同一性程度从98 - 99%（13C280、GYV - 2014）到80%（Rasp1 - 2014）不等。系统发育研究揭示了Rasp1 - 201 RNA1和RNA2的不同起源，表明存在重配现象。在RNA1的3'UTR和5'端区域还鉴定出了两个重组事件。
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