Zhao Xiyan, Anane Rex Frimpong, Guo Jianwei, Zi Shaomei, Chu Bifan, Yang Zefen, Yan Kai, Ji Kaifei, Li Shangyun, Chen Zeli, Zhao Mingfu
Key Laboratory for Agricultural Bioaffiliationersity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, NO. 95, Jinhei Road, Panlong District, Kunming, Yunnan, 650201, China.
Monash Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, 23 Innovation Walk, Clayton, VIC, 3800, Australia.
Arch Virol. 2024 Dec 12;170(1):10. doi: 10.1007/s00705-024-06196-z.
Here, we report the complete genome sequence of Jasminum polyanthum nepovirus 1 (JPV1), a novel virus associated with foliar ringspot symptoms in Jasminum polyanthum (Oleaceae). The genome of JPV1 consists of RNA1 and RNA2, both of which are monocistronic and are 7081 nt and 4212 nt in length, respectively. RNA1 encodes six functional proteins, including the X1 protein, X2 protein, NTP-binding protein (NTB), VPg protein, protease cofactor (Pro), and RNA-dependent RNA polymerase (RdRp), while RNA2 encodes three functional proteins: the 2A protein, movement protein (MP), and capsid protein (CP). Pairwise sequence comparisons showed that the JPV1 protease-polymerase (Pro-Pol) and CP proteins each shared less than 40% aa sequence identity with those of other nepoviruses. Phylogenetic analysis indicated that JPV1 is a putative member of the genus Nepovirus. Based on these results, we propose that JPV1 is a member of subgroup B of the genus Nepovirus.
在此，我们报道了多花素馨潜隐病毒1（JPV1）的全基因组序列，该病毒是一种与多花素馨（木犀科）叶环斑症状相关的新型病毒。JPV1的基因组由RNA1和RNA2组成，二者均为单顺反子，长度分别为7081 nt和4212 nt。RNA1编码六种功能蛋白，包括X1蛋白、X2蛋白、NTP结合蛋白（NTB）、VPg蛋白、蛋白酶辅因子（Pro）和RNA依赖的RNA聚合酶（RdRp），而RNA2编码三种功能蛋白：2A蛋白、运动蛋白（MP）和衣壳蛋白（CP）。成对序列比较显示，JPV1的蛋白酶-聚合酶（Pro-Pol）和CP蛋白与其他潜隐病毒的相应蛋白的氨基酸序列同一性均低于40%。系统发育分析表明，JPV1是潜隐病毒属的一个假定成员。基于这些结果，我们提出JPV1是潜隐病毒属B亚组的一个成员。
