Westermann J, Ronneberg S, Fritz F J, Pabst R
Center of Anatomy, Medical School of Hannover, FRG.
Eur J Immunol. 1989 Jun;19(6):1087-93. doi: 10.1002/eji.1830190619.
Adult, male Lewis rats received a single injection of 5-bromo-2'-deoxyuridine (BrdUrd) i.v. to label proliferating cells in the S phase of the cell cycle. After 1 and 24 h the thymus, bone marrow, blood, spleen, peripheral, cervical and mesenteric lymph nodes as well as Peyer's patches were removed. In cell suspensions surface staining was performed for B, T, T helper (Th) and cytotoxic/suppressor (Tc/s) T lymphocytes by identifying kappa light chain, CD5+, CD4+ and CD8+ cells, respectively. On the same slide the DNA label BrdUrd was demonstrated by a monoclonal antibody. B, T, Th and Tc/s lymphocytes proliferate locally both in central lymphoid organs such as the thymus and the bone marrow, and in peripheral lymphoid organs such as the spleen, lymph nodes and Peyer's patches. Within an organ the amount of proliferation among the lymphocyte subsets is similar, differing not more than threefold. Although concerning only a small fraction of cells within the organ, an unexpected finding is the high percentage of BrdUrd-labeled cells among B lymphocytes in the thymus (3%) and among T lymphocytes in the bone marrow (3%). One day after injection of BrdUrd the thymus contains 25% BrdUrd+ T lymphocytes, while the other organs investigated do not show more than about 2% BrdUrd+ B and T lymphocytes. Many of the newly formed lymphocyte subsets leave their organ of birth within 24 h. Thus the amount of proliferation in the lymphocyte subsets investigated is very similar and the differences between central (thymus and bone marrow) and peripheral lymphoid organs are much smaller than expected.
成年雄性Lewis大鼠静脉注射一次5-溴-2'-脱氧尿苷（BrdUrd），以标记细胞周期S期的增殖细胞。注射后1小时和24小时，取出胸腺、骨髓、血液、脾脏、外周、颈部和肠系膜淋巴结以及派尔集合淋巴结。在细胞悬液中，通过分别鉴定κ轻链、CD5 +、CD4 +和CD8 +细胞，对B淋巴细胞、T淋巴细胞、辅助性T（Th）淋巴细胞和细胞毒性/抑制性（Tc/s）T淋巴细胞进行表面染色。在同一张载玻片上，用单克隆抗体检测DNA标记物BrdUrd。B淋巴细胞、T淋巴细胞、Th淋巴细胞和Tc/s淋巴细胞在中枢淋巴器官如胸腺和骨髓以及外周淋巴器官如脾脏、淋巴结和派尔集合淋巴结中均有局部增殖。在一个器官内，淋巴细胞亚群之间的增殖量相似，差异不超过三倍。尽管仅涉及器官内一小部分细胞，但一个意外发现是胸腺中B淋巴细胞（3%）和骨髓中T淋巴细胞（3%）中BrdUrd标记细胞的比例很高。注射BrdUrd一天后，胸腺中含有25%的BrdUrd + T淋巴细胞，而其他被研究的器官中BrdUrd + B淋巴细胞和T淋巴细胞不超过约2%。许多新形成的淋巴细胞亚群在24小时内离开其诞生器官。因此，所研究的淋巴细胞亚群中的增殖量非常相似，中枢（胸腺和骨髓）和外周淋巴器官之间的差异比预期小得多。
