中枢和外周听觉系统均参与大鼠水杨酸盐诱导的耳鸣：一项行为学研究。
Both central and peripheral auditory systems are involved in salicylate-induced tinnitus in rats: a behavioral study.
作者信息
Chen Guanyin, Feng Lining, Liu Zhi, Sun Yongzhu, Chang Haifeng, Cui Pengcheng
机构信息
Department of Otolaryngology Head and Neck Surgery, Tangdu Hospital, the Fourth Military Medical University, Xi'an, China.
Department of Clinical Medicine, Faculty of Aerospace medicine, the Fourth Military Medical University, Xi'an, China.
出版信息
PLoS One. 2014 Sep 30;9(9):e108659. doi: 10.1371/journal.pone.0108659. eCollection 2014.
OBJECTIVE
This study was designed to establish a low dose salicylate-induced tinnitus rat model and to investigate whether central or peripheral auditory system is involved in tinnitus.
METHODS
Lick suppression ratio (R), lick count and lick latency of conditioned rats in salicylate group (120 mg/kg, intraperitoneally) and saline group were first compared. Bilateral auditory nerves were ablated in unconditioned rats and lick count and lick latency were compared before and after ablation. The ablation was then performed in conditioned rats and lick count and lick latency were compared between salicylate group and saline group and between ablated and unablated salicylate groups.
RESULTS
Both the R value and the lick count in salicylate group were significantly higher than those in saline group and lick latency in salicylate group was significantly shorter than that in saline group. No significant changes were observed in lick count and lick latency before and after ablation. After ablation, lick count and lick latency in salicylate group were significantly higher and shorter respectively than those in saline group, but they were significantly lower and longer respectively than those in unablated salicylate group.
CONCLUSION
A low dose of salicylate (120 mg/kg) can induce tinnitus in rats and both central and peripheral auditory systems participate in the generation of salicylate-induced tinnitus.
目的
本研究旨在建立低剂量水杨酸盐诱导的耳鸣大鼠模型，并研究耳鸣是否涉及中枢或外周听觉系统。
方法
首先比较水杨酸盐组（120mg/kg，腹腔注射）和生理盐水组中条件大鼠的舔抑制率（R）、舔舐次数和舔舐潜伏期。对未条件化大鼠双侧听神经进行切断，并比较切断前后的舔舐次数和舔舐潜伏期。然后对条件化大鼠进行切断，并比较水杨酸盐组和生理盐水组之间以及切断和未切断的水杨酸盐组之间的舔舐次数和舔舐潜伏期。
结果
水杨酸盐组的R值和舔舐次数均显著高于生理盐水组，且水杨酸盐组的舔舐潜伏期显著短于生理盐水组。切断前后舔舐次数和舔舐潜伏期均未观察到显著变化。切断后，水杨酸盐组的舔舐次数和舔舐潜伏期分别显著高于和短于生理盐水组，但分别显著低于和长于未切断的水杨酸盐组。
结论
低剂量水杨酸盐（120mg/kg）可诱导大鼠耳鸣，中枢和外周听觉系统均参与水杨酸盐诱导耳鸣的产生。
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