Farhadi Mohammad, Gorji Ali, Mirsalehi Marjan, Müller Marcus, Poletaev Alexander Borisovich, Mahboudi Fereidoun, Asadpour Abdoreza, Ebrahimi Mohammad, Beiranvand Mohaddeseh, Khaftari Mohaddeseh Dehghani, Akbarnejad Zeinab, Mahmoudian Saeid
ENT and Head and Neck Research Center, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Epilepsy Research Center, Department of Neurosurgery Westfälische Wilhelms-Universitat Münster, Münster, Germany.
Heliyon. 2023 Aug 11;9(8):e19052. doi: 10.1016/j.heliyon.2023.e19052. eCollection 2023 Aug.
The effect of neuroprotective placental protein composition (NPPC) on the suppression of tinnitus and the restoration of the auditory brainstem response (ABR) characteristics was explored in tinnitus-induced rats. The animals were placed into two groups: (1) the study group, rats received sodium salicylate (SS) at the dose of 200 mg/kg twice a day for two weeks, and then 0.4 mg of the NPPC per day, between the 14th and 28th days, (2) the placebo group, rats received saline for two weeks, and then the NPPC alone between the 14th and 28th days. The gap pre-pulse inhibition of the acoustic startle (GPIAS), the pre-pulse inhibition (PPI), and the ABR assessments were performed on animals in both groups three times (baseline, day 14, and 28). The GPIAS value declined after 14 consecutive days of the SS injection, while NPPC treatment augmented the GPIAS score in the study group on the 28th day. The PPI outcomes revealed no significant changes, indicating hearing preservation after the SS and NPPC administrations. Moreover, some changes in ABR characteristics were observed following SS injection, including (1) higher ABR thresholds, (2) lowered waves I and II amplitudes at the frequencies of 6, 12, and 24 kHz and wave III at the 12 kHz, (3) elevated amplitude ratios, and (4) prolongation in brainstem transmission time (BTT). All the mentioned variables returned to their normal values after applying the NPPC. The NPPC use could exert positive therapeutic effects on the tinnitus-induced rats and improve their ABR parameters.
在耳鸣诱导的大鼠中，研究了神经保护胎盘蛋白组合物（NPPC）对耳鸣抑制和听觉脑干反应（ABR）特征恢复的影响。将动物分为两组：（1）研究组，大鼠每天两次接受200mg/kg剂量的水杨酸钠（SS），持续两周，然后在第14天至28天期间每天接受0.4mg的NPPC；（2）安慰剂组，大鼠接受两周的生理盐水，然后在第14天至28天期间仅接受NPPC。对两组动物进行三次听觉惊吓间隙前脉冲抑制（GPIAS）、前脉冲抑制（PPI）和ABR评估（基线、第14天和第28天）。连续14天注射SS后，GPIAS值下降，而在第28天，NPPC治疗使研究组的GPIAS评分增加。PPI结果显示无显著变化，表明SS和NPPC给药后听力得以保留。此外，注射SS后观察到ABR特征有一些变化，包括：（1）ABR阈值升高；（2）在6、12和24kHz频率下I波和II波振幅降低，在12kHz频率下III波振幅降低；（3）振幅比升高；（4）脑干传导时间（BTT）延长。应用NPPC后，所有上述变量均恢复到正常水平。使用NPPC可对耳鸣诱导的大鼠产生积极的治疗效果，并改善其ABR参数。
