Guo Xuena, Liu Wei, Bai Xuejing, He Xiuping, Zhang Borun
CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
World J Microbiol Biotechnol. 2014 Dec;30(12):3245-50. doi: 10.1007/s11274-014-1751-8. Epub 2014 Oct 1.
High-performance liquid chromatography was used to separate Cr(III) and Cr(VI) in samples with detection by inductively coupled plasma mass spectrometry(ICP-MS). The separation was achieved on a weak anion exchange column. The mobile phase was pH 7.0 ammonium nitrate solution. The redox reaction between Cr(III) and Cr(VI) was avoided during separation and determination. This separation method could be used to separate the samples with large concentration differences between Cr(III) and Cr(VI). The alkaline digestion was used to extract chromium in solid sample, which had no effect on the retention time and the peak area of the Cr(VI). However, the conversion of Cr(VI) from Cr(III) was observed during alkaline digestion, which displayed positive relation with the ratio of Cr(III) and Cr(VI) in samples. Both Cr(III) and Cr(VI) contents of chromium yeasts cultured in media with different chromium additions were determined. The spike recoveries of Cr(VI) for chromium yeasts were in the range of 95-108 %.
采用高效液相色谱法在弱阴离子交换柱上分离样品中的Cr(III)和Cr(VI)，并通过电感耦合等离子体质谱(ICP-MS)进行检测。流动相为pH 7.0的硝酸铵溶液。在分离和测定过程中避免了Cr(III)和Cr(VI)之间的氧化还原反应。该分离方法可用于分离Cr(III)和Cr(VI)浓度差异较大的样品。采用碱消解法提取固体样品中的铬，该方法对Cr(VI)的保留时间和峰面积没有影响。然而，在碱消解过程中观察到Cr(III)向Cr(VI)的转化，且该转化与样品中Cr(III)和Cr(VI)的比例呈正相关。测定了在添加不同铬的培养基中培养的铬酵母中Cr(III)和Cr(VI)的含量。铬酵母中Cr(VI)的加标回收率在95-108%范围内。
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